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首页> 外文期刊>Frontiers in Veterinary Science >Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle
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Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle

机译：Neospora caninum微肿瘤蛋白26的表征及其潜力用作牛新孢子症的诊断标志物

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The apicomplexan parasite Neospora caninum causes neosporosis, an illness that leads to abortion or stillbirth in cattle, causing massive economic losses to the livestock industry. Rapid and viable diagnosis is the premise of prevention and control for neosporosis. In this study, we screened a new microneme protein 26 (NcMIC26) through Western blot and mass spectrometry identification from the excretory secretion antigen (ESA) of N. caninum tachyzoites. NcMIC26 is subcellularly localized to the microneme of parasites. NcMIC26 is a specific antigen of N. caninum and has no cross-reaction with Toxoplasma gondii. Therefore, NcMIC26 has the potential to be a candidate diagnostic antigen for neosporosis. To test this hypothesis, recombinant NcMIC26 (rNcMIC26) was expressed in Escherichia. coli, and an indirect ELISA for detecting anti-N. caninum antibodies in cattle was established. Compared with that of the Indirect Immunofluorescent Antibody Test (IFAT), the positive coincidence rate of the ELISA based on rNcMIC26 was 76.53% (75/98), which was higher than that of an ELISA based on rSRS2 (66.33%), and the negative coincidence rate was 84.62% (33/39). It is noteworthy that 36 positive samples confirmed by IFAT were consistent with the rNcMIC26 ELISA but were negative by the rNcSRS2 ELISA. Our research illustrated that NcMIC26 is a dependable diagnostic marker for the serodiagnosis of N. caninum infection in cattle and could be utilized as a supplementary antigen for missed detection by NcSRS2.
机译：ApiCoMplexan寄生虫Neospora caninum导致新孢子症，一种导致牛流产或死产的疾病，导致畜牧业的巨大经济损失。快速和可行的诊断是预防和控制新孢子症的前提。在这项研究中，我们通过N罐抗凝固抗原（ESA）的蛋白质印迹和质谱鉴定筛选了一种新的微量蛋白质26（NCMIC26）。 NCMIC26亚细胞定位于寄生虫的微米。 NCMIC26是N罐的特异性抗原，并且没有与弓形虫的交叉反应。因此，NCMIC26具有候选诊断抗原的次孢子症。为了测试该假设，重组NCMIC26（RNCMIC26）在大肠杆菌中表达。 Coli，以及用于检测抗-N的间接ELISA。牛的甘油抗体建立。与间接免疫荧光抗体试验（IFAT）的相比，基于RNCMIC26的ELISA的正刚性率为76.53％（75/98），其基于RSRS2（66.33％），以及负甘露率为84.62％（33/39）。值得注意的是，IFAT证实36种阳性样品与RNCMIC26 ELISA一致，但rncsrs2 elisa是阴性的。我们的研究表明，NCMIC26是牛牛粪感染的血型可靠诊断标志物，并且可以用作NCSRS2未错过检测的补充抗原。

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