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Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content

机译：改变污泥磷含量的生物磷酸盐去除系统的微生物群落变化

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Biomarkers (respiratory quinones and cellular fatty acids) and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA genes were used to characterize the microbial community structure of lab-scale enhanced biological phosphate-removal (EBPR) systems in response to altering sludge phosphorus (P) content. All the data suggest that the microbial community structures of sludge samples with a P content between 8 and 12·3% (sludge dry weight) (i.e. good EBPR activity) were very similar, but differed from those with 2% P content (i.e. no EBPR activity). For all samples analysed, ubiquinones Q-8 and Q-10, menaquinone MK-8(H4), and fatty acids C16:0, C16:1ω9cand C18:1ω11cwere the major components. The dominance of Q-8, Q-10 and MK-8(H4) suggested that large numbers of organisms belonging to the β and α subclasses of the Proteobacteria and the Actinobacteria from the high G+C Gram-positive bacteria, respectively, were present. DGGE analysis revealed at least 7–9 predominant DNA bands and numerous other fragments in each sample. Five major DGGE fragments from each of the 2% and 12% P-containing sludge samples, respectively, were successfully isolated and sequenced. Phylogenetic analysis of the sequences indicated that both 2% and 12% P-containing sludge samples shared three common phylotypes that were separately affiliated with a novel bacterial group from the γ subclass of the Proteobacteria, two MK-8(H4)-containing actinobacteria previously isolated from the 2% P-containing sludge, and a Caulobacter spp. in the α subclass of the Proteobacteria. The phylogenetic analysis also revealed phylotypes unique to both sludge samples. Changes in sludge P content therefore had an effect on the composition and abundance of the predominant microbial populations, though specific phylotypes could not be unequivocally associated with EBPR.
机译：PCR扩增的16S rRNA基因的生物标志物（呼吸醌和细胞脂肪酸）和变性梯度凝胶电泳（DGGE）用于表征实验室规模增强的生物磷酸盐除去（EBPR）系统的微生物群落结构，响应于改变污泥磷（P）含量。所有数据表明，污泥样品的微生物群落结构具有8-12·3％（污泥干重）（即良好的EBPR活性）非常相似，但与具有2％P含量的污泥（即， EBPR活动）。对于分析的所有样品，泛醌Q-8和Q-10，Menaquinone MK-8（H4）和脂肪酸C16：0，C16：1ω9CANDC18：1Ω11CWE。 Q-8，Q-10和MK-8（H4）的主导地位表明，分别属于β和α亚类和来自高G + C革兰氏阳性细菌的β和α亚类的大量生物体展示。 DGGE分析显示至少7-9个主要的DNA带和每个样品中的许多其他碎片。成功分离和测序来自2％和12％含P含P污泥样品中的每一个的五个主要DGGE片段。序列的系统发育分析表明，2％和12％的含P污泥样品共用三种常见的植物，其与来自植物的γ-8（H4） - 甲基菌的γ-8（H4）癌的γ-8（H4） - 抗菌杀菌剂分别隶属于与含2％含P的污泥分离，以及蛭杆菌SPP。在蛋白的α子类中。系统发育分析还揭示了污泥样品所特有的植物型。因此，污泥P含量的变化对主要的微生物群体的组成和丰度具有效果，尽管特异性植物不能与EBPR无概念。

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