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首页> 外文期刊>Microbiology >Biosynthesis of the unusual 5,5-gem-dimethyl-deoxysugar noviose: investigation of the C-methyltransferase gene cloU
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Biosynthesis of the unusual 5,5-gem-dimethyl-deoxysugar noviose: investigation of the C-methyltransferase gene cloU

机译：异合物的异合剂的5,5-吉甲基 - 脱氧瘤Noviose：C-甲基转移酶基因CLOU的研究

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摘要

The aminocoumarin antibiotic clorobiocin contains an unusual branched deoxysugar with a 5,5-gem-dimethyl structure. Inactivation of the putative C-methyltransferase gene cloU was carried out, which led to the loss of the axial methyl group at C-5 of this deoxysugar moiety. This result establishes the function of cloU, and at the same time it proves that the biosynthesis of the deoxysugar moiety of clorobiocin proceeds via a 3,5-epimerization of the dTDP-4-keto-6-deoxyglucose intermediate. The inactivation was carried out on a cosmid which contained the entire clorobiocin biosynthetic gene cluster. Expression of the modified cluster in a heterologous host led to the formation of desmethyl-clorobiocin and a structural isomer thereof. Both compounds were isolated on a preparative scale, their structures were elucidated by 1H-NMR and mass spectroscopy and their antibacterial activity was assayed.
机译：Aminocoumarin抗生素Clorobiocin含有一个不寻常的分支脱氧糖糖，具有5,5-珠宝二甲基结构。进行了调用的C-甲基转移酶基因CLOU的失活，其导致该脱氧谷部分C-5处的轴向甲基的损失。该结果建立了CLOU的功能，同时证明了Clorociocin的DTOxysugar部分的生物合成通过DTDP-4-酮-6-脱氧葡萄糖中间体的3,5-缩影进行。灭活是在含有整个克罗布二霉素生物合成基因簇的粘性体中进行的。改性簇在异源宿主中的表达导致形成去甲基 - 克罗双霉素及其结构异构体。两种化合物在制备规模上分离，其结构通过1H-NMR和质谱，测定它们的抗菌活性。

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