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首页> 外文期刊>Microbiology >Hierarchical control of virginiamycin production in Streptomyces virginiae by three pathway-specific regulators: VmsS, VmsT and VmsR
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Hierarchical control of virginiamycin production in Streptomyces virginiae by three pathway-specific regulators: VmsS, VmsT and VmsR

机译：三种途径调节器中弗吉尼亚昔甙生产的弗吉尼亚霉素生产的层次控制：VMS，VMST和VMSR

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Two regulatory genes encoding a Streptomyces antibiotic regulatory protein (vmsS) and a response regulator (vmsT) of a bacterial two-component signal transduction system are present in the left-hand region of the biosynthetic gene cluster of the antibiotic virginiamycin, which is composed of virginiamycin M (VM) and virginiamycin S (VS), in Streptomyces virginiae. Disruption of vmsS abolished both VM and VS biosynthesis, with drastic alteration of the transcriptional profile for virginiamycin biosynthetic genes, whereas disruption of vmsT resulted in only a loss of VM biosynthesis, suggesting that vmsS is a pathway-specific regulator for both VM and VS biosynthesis, and that vmsT is a pathway-specific regulator for VM biosynthesis alone. Gene expression profiles determined by semiquantitative RT-PCR on the virginiamycin biosynthetic gene cluster demonstrated that vmsS controls the biosynthetic genes for VM and VS, and vmsT controls unidentified gene(s) of VM biosynthesis located outside the biosynthetic gene cluster. In addition, transcriptional analysis of a deletion mutant of vmsR located in the clustered regulatory region in the virginiamycin cluster (and which also acts as a SARP-family activator for both VM and VS biosynthesis) indicated that the expression of vmsS and vmsT is under the control of vmsR, and vmsR also contributes to the expression of VM and VS biosynthetic genes, independent of vmsS and vmsT. Therefore, coordinated virginiamycin biosynthesis is controlled by three pathway-specific regulators which hierarchically control the expression of the biosynthetic gene cluster.
机译：编码抗生素双组分信号转导系统的链霉菌抗生素调节蛋白（VMS）和响应调节剂（VMST）的两种调节基因存在于抗生素维尼昔亚霉素的生物合酶基因群的左侧区域中Virginiamycin M（VM）和Virginiamycin S（VS），在Streptomyces弗吉尼亚岛。 VMS的破坏消除了VM和VS生物合成，随着VMS的转录谱的急剧改变，而VMST的破坏导致VM生物合成的丧失，表明VMS是VM和VS生物合成的途径特异性调节因子，并且VMST是一种特定于VM生物合成的途径调节器。通过弗吉尼亚霉素生物合成基因簇上的半定量RT-PCR确定的基因表达谱证明了VMS对VM和VS的生物合成基因，并且VMST对位于生物合成基因簇外的VM生物合成的未识别的基因。此外，位于弗吉尼亚霉素簇中的聚类调节区的VMSR缺失突变体的转录分析（其也用作VM和VS生物合成的SARP-Family Activator）表明VMS和VMST的表达是下方的VMSR的控制，VMSR也有助于表达VM和VS生物合成基因，与VMS和VMST无关。因此，协调的维尔尼霉素生物合成由三个途径特异性调节剂控制，该途径对生物合成基因簇的表达进行了层次。

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