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首页> 外文期刊>Microbiology >nuvA, An Aspergillus Nidulans Gene Involved in DNA Repair and Recombination, is a Homologue of Saccharomyces Cerevisiae RAD18 and Neurospora Crassa uvs-2
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nuvA, An Aspergillus Nidulans Gene Involved in DNA Repair and Recombination, is a Homologue of Saccharomyces Cerevisiae RAD18 and Neurospora Crassa uvs-2

机译：Nuva，涉及DNA修复和重组的曲霉属植物基因，是酿酒酵母酿酒酵母的同源物和Neurospora Crassa UVS-2的同源物

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A 40 kb genomic clone and 2.3 kb EcoRI subclone that rescued the DNA repair and recombination defects of the Aspergillus nidulans nuvA11 mutant were isolated and the subclone sequenced. The subclone hybridized to a cosmid in a chromosome-specific library confirming the assignment of nuvA to linkage group IV and indicating its closeness to bimD. Amplification by PCR clarified the relative positions of nuvA and bimD. A region identified within the subclone, encoding a C3HC4 zinc finger motif, was used as a probe to retrieve a cDNA clone. Sequencing of this clone showed that the nuvA gene has an ORF of 1329 bp with two introns of 51 bp and 60 bp. Expression of nuvA appears to be extremely low. The putative NUVA polypeptide has two zinc finger motifs, a molecular mass of 48906 Da and has 39% identity with the Neurospora crassa uvs-2 and 25% identity with the Saccharomyces cerevisiae RAD18 translation products. Although mutations in nuvA, uvs-2 and RAD18 produce similar phenotypes, only the nuvA11 mutation affects meiotic recombination. A role for nuvA in both DNA repair and genetic recombination is proposed.
机译：40 kB基因组克隆和2.3 kB生态亚克酮，用于分离出DNA修复和复合缺陷的DNA修复和重组缺陷，并分离出亚旋钮测序。将亚旋钮杂交至染色体特异性文库中的助核，证实Nuva分配联动IV族，并表明其对BIMD的亲密度。 PCR扩增阐明了NUVA和BIMD的相对位置。在编码C3HC4锌手指基序的亚克星内鉴定的区域用作检索cDNA克隆的探针。该克隆的测序显示Nuva基因具有1329bp的ORF，具有51bp和60bp的两个内含子。 Nuva的表达似乎极低。推定的Nuva多肽具有两个锌手指基序，分子量为48906Da，与神经孢子Crassa UVS-2和25％同一性，与酿酒酵母的酿酒酵母Rad18翻译产品具有39％的同一性。虽然NuvA，UVS-2和RAD18中的突变产生了类似的表型，但Nuva11突变只会影响减数分裂重组。提出了对DNA修复和遗传重组的NUVA的作用。

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