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首页> 外文期刊>Microbiology >Analysis of σ54-dependent genes in Enterococcus faecalis: a mannose PTS permease (EIIMan) is involved in sensitivity to a bacteriocin, mesentericin Y105
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Analysis of σ54-dependent genes in Enterococcus faecalis: a mannose PTS permease (EIIMan) is involved in sensitivity to a bacteriocin, mesentericin Y105

机译：肠球菌粪便中σ54依赖性基因分析：甘露糖PTS允许（EIIMAN）涉及对细菌霉素的敏感性，Mesentiricin Y105

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The σ54 RNA polymerase subunit has a prominent role in susceptibility of Listeria monocytogenes and Enterococcus faecalis to mesentericin Y105, a class IIa bacteriocin. Consequently, σ54-dependent genes as well as specific activators also required for expression of these genes were sought. Five putative σ54-associated activators were detected in the genome of E. faecalis V583, and all but one could activate the transcription of permease genes belonging to sugar phosphotransferase systems (PTSs). Interestingly, these activators display a helicase signature not yet reported in this activator family, which could explain the ATP-dependent mechanism of DNA unwinding preceding the start of transcription. To find which activator is linked to susceptibility of E. faecalis to mesentericin Y105, their respective genes were subsequently interrupted. Among them, only mptR gene interruption led to a resistance phenotype. Immediately downstream from mptR, a putative σ54-dependent operon was found to encode a mannose PTS permease, namely (EII_{t}^{Man}) . Moreover, in liquid culture, glucose and mannose induced the sensitivity of E. faecalis to mesentericin Y105. Since sugars have previously been reported to induce PTS permease expression, it appears that (EII_{t}^{Man}) expression, presumably induced in the presence of glucose and mannose, leads to an enhanced sensitivity of E. faecalis to the bacteriocin. Additional information was gained from knockouts within the permease operon. Interruption of the distal mptD gene, which encodes the IID subunit of (EII_{t}^{Man}) , strikingly led to resistance to mesentericin Y105. Moreover, MptD appears to be a peculiar membrane subunit, bearing an additional domain compared to most known IID subunits. According to these results, (EII_{t}^{Man}) is clearly involved in susceptibility to mesentericin Y105 and could even be its receptor at the E. faecalis surface. Finally, it is hypothesized that MptD could be responsible for the targeting specificity, via an interaction between its additional domain and mesentericin Y105.
机译：σ54RNA聚合酶亚基在李斯特菌单核细胞增生和肠球菌粪便雌激素对史氏菌，塞森霉素Y105，A类IIA菌菌，具有突出作用。因此，寻求Σ54依赖性基因以及还需要表达这些基因所需的特异性活化剂。在E. faecalis v583的基因组中检测到五个推定的σ54-相关活化剂，除了一个，也可以激活属于糖磷酸转移酶系统（PTS）的允许基因的转录。有趣的是，这些活化剂显示出在该激活剂家族中尚未报道的螺旋酶签名，其可以解释在转录开始之前的DNA展开的ATP依赖机制。为了发现哪些激活剂与E.粪便的易感性联系到Mesentericin Y105，随后中断它们各自的基因。其中，只有MPTR基因中断导致阻力表型。立即从MPTR下游，发现一个推定的Σ54依赖的操纵子编码甘露糖PTS允许，即（eii_ {t} ^ {man} ）。此外，在液体培养物中，葡萄糖和甘露糖诱导E.粪便患者对史塞霉素Y105的敏感性。由于先前据报道糖诱导PTS允许表达，因此似乎可能在葡萄糖和甘露糖存在下诱导的（EII_ {T} ^ {MAN} ）表达，导致E. FAECALIS的增强敏感性细菌霉素。允许倍转操纵子内的淘汰赛中获得了其他信息。对远端MPTD基因的中断，它们编码（eii_ {t ^ {man}}的IID亚基，尖锐地导致抗胸膜素蛋白Y105。此外，MPTD似乎是一种特殊的膜亚基，与最着名的IID亚基相比，额外的域。根据这些结果，（eii_ {t} ^ {man}）显然涉及骨髓素蛋白Y105的易感性，甚至可以是其E.粪便表面的受体。最后，假设MPTD可以通过其附加领域和肠系霉素Y105之间的相互作用对靶向特异性负责。

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