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首页> 外文期刊>Microbiology >The autophagy gene BbATG5, involved in the formation of the autophagosome, contributes to cell differentiation and growth but is dispensable for pathogenesis in the entomopathogenic fungus Beauveria bassiana
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The autophagy gene BbATG5, involved in the formation of the autophagosome, contributes to cell differentiation and growth but is dispensable for pathogenesis in the entomopathogenic fungus Beauveria bassiana

机译：涉及自噬瘤形成的自噬基因Bbatg5有助于细胞分化和生长，但是昆虫疗法真菌Beauveria bassiana的发病机会是可分配的

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摘要

Autophagy is a highly conserved process, representing the major eukaryotic degradative pathway of cellular components. Autophagy-mediated recycling of cellular materials contributes to cell differentiation, tissue remodelling and proper development. In fungi, autophagy is required for normal growth and cell differentiation. The entomopathogenic fungus Beauveria bassiana and its invertebrate targets represent a unique model system with which to examine host–pathogen interactions. The ATG5 gene is one of 17 involved in autophagosome formation, and the B. bassiana homologue (BbATG5) was identified. The role of autophagy in B. bassiana growth and virulence was investigated via construction of a targeted gene knockout of BbATG5. The mutant strain displayed increased sensitivity to nutrient limitation, with decreased germination and growth as compared with the wild-type parent. Conidiation was severely compromised and conidia derived from the ΔBbATG5 strain were altered in morphology. Cell differentiation into blastospores was also greatly reduced. Despite the significant growth and developmental defects, insect bioassays using the oriental leafworm moth, Spodoptera litura, indicated a modest (~40?%) decrease in virulence in the ΔBbATG5 strain. The phenotypic defects of the ΔBbATG5 strain could be restored by introduction of an intact copy of BbATG5. These data suggest that unlike several plant and animal pathogenic fungi, where ATG5 is required for infection, in B. bassiana it is dispensable for pathogenesis.
机译：自噬是一种高度保守的过程，代表细胞组分的主要真核降解途径。自噬介导的细胞材料的再循环有助于细胞分化，组织重塑和适当的发育。在真菌中，正常生长和细胞分化需要自噬。昆虫疗法菌嗜孢菌贝斯氏菌和其无脊椎动物靶标代表了一种独特的模型系统，用于检查宿主病原体相互作用。 ATG5基因是Autophosome形成中参与的17个，鉴定了B.Bassiana同源物（BBATG5）。通过建设BBATG5的靶向基因敲除，研究了自噬在B.Bassiana生长和毒力中的作用。突变菌株显示对营养率限制的敏感性增加，与野生型母体相比，萌发和生长降低。结合被严重损害，并且衍生自ΔBbatg5菌株的分类在形态中改变。细胞分化进入胚胎孢子也大大降低。尽管具有显着的增长和发育缺陷，但是使用东方叶虫蛾的昆虫生物测量，Spodoptera Litura表明ΔBbatg5菌株中的毒力量的温和（〜40倍）降低。通过引入BBATG5的完整拷贝，可以恢复ΔBbatg5菌株的表型缺陷。这些数据表明，与几种植物和动物致病真菌不同，在感染时需要ATG5，Basiana在Bassiana中可分配发病机制。

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