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首页> 外文期刊>Microorganisms >Subcellular Location of Piscirickettsia salmonis Heat Shock Protein 60 (Hsp60) Chaperone by Using Immunogold Labeling and Proteomic Analysis
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Subcellular Location of Piscirickettsia salmonis Heat Shock Protein 60 (Hsp60) Chaperone by Using Immunogold Labeling and Proteomic Analysis

机译：Piscirickettsia Salmonis热休克蛋白60（HSP60）伴随免疫标记和蛋白质组学分析的亚细胞位置

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Piscirickettsia salmonis is the causative bacterial agent of piscirickettsiosis, a systemic fish disease that significantly impacts the Chilean salmon industry. This bacterium possesses a type IV secretion system (T4SS), several proteins of the type III secretion system (T3SS), and a single heat shock protein 60 (Hsp60/GroEL). It has been suggested that due to its high antigenicity, the P. salmonis Hsp60 could be surface-exposed, translocated across the membrane, and (or) secreted into the extracellular matrix. This study tests the hypothesis that P. salmonis Hsp60 could be located on the bacterial surface. Immunogold electron microscopy and proteomic analyses suggested that although P. salmonis Hsp60 was predominantly associated with the bacterial cell cytoplasm, Hsp60-positive spots also exist on the bacterial cell envelope. IgY antibodies against P. salmonis Hsp60 protected SHK-1 cells against infection. Several bioinformatics approaches were used to assess Hsp60 translocation by the T4SS, T3SS, and T6SS, with negative results. These data support the hypothesis that small amounts of Hsp60 must reach the bacterial cell surface in a manner probably not mediated by currently characterized secretion systems, and that they remain biologically active during P. salmonis infection, possibly mediating adherence and (or) invasion.
机译：Piscirickettsia Salmonis是Piscirickettsiosis的致病细菌剂，一种系统性鱼类疾病，显着影响了智利鲑鱼行业。该细菌具有IV型分泌系统（T4SS），III型分泌系统（T3S）的几种蛋白质，以及单个热休克蛋白60（Hsp60 / Groel）。已经提出，由于其高抗原性，P.Salmonis HSP60可以表面暴露，穿过膜，（或）分泌到细胞外基质中。该研究测试了P. salmonis Hsp60可以位于细菌表面上的假设。免疫角膜电子显微镜和蛋白质组学分析表明，尽管P. Salmonis Hsp60主要与细菌细胞细胞质相关，但细菌细胞包膜上也存在HSP60阳性斑点。针对P. Salmonis HSP60的IgY抗体保护SHK-1细胞免受感染。几种生物信息学方法用于评估T4S，T3S和T6SS的HSP60易位，具有负结果。这些数据支持假设，即少量HSP60必须以可能未被目前特征的分泌系统介导的方式到达细菌细胞表面，并且它们在P. Salmonis感染期间保持生物活性，可能介导粘附和（或）侵袭。

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