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首页> 外文期刊>Neoplasia: an international journal for oncology research >Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma
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Isolation of Human CD138+ Microparticles from the Plasma of Patients with Multiple Myeloma

机译：从多种骨髓瘤患者的血浆中分离人CD138 +微粒

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The confinement of multiple myeloma (MM) to the bone marrow microenvironment requires an invasive bone marrow biopsy to monitor the malignant compartment. The existing clinical tools used to determine treatment response and tumor relapse are limited in sensitivity mainly because they indirectly measure tumor burden inside the bone marrow and fail to capture the patchy, multisite tumor infiltrates associated with MM. Microparticles (MPs) are 0.1- to 1.0-μm membrane vesicles, which contain the cellular content of their originating cell. MPs are functional mediators and convey prothrombotic, promalignant, proresistance, and proinflammatory messages, establishing intercellular cross talk and bypassing the need for direct cell-cell contact in many pathologies. In this study, we analyzed plasma cell–derived MPs (CD138+) from deidentified MM patients (n = 64) and normal subjects (n = 18) using flow cytometry. The morphology and size of the MPs were further analyzed using scanning electron microscopy. Our study shows the proof of a systemic signature of MPs in MM patients. We observed that the levels of MPs were significantly elevated in MM corresponding to the tumor burden. We provide the first evidence for the presence of MPs in the peripheral blood of MM patients with potential applications in personalized MM clinical monitoring.
机译：多发性骨髓瘤（mm）对骨髓微环境的限制需要侵入性骨髓活检以监测恶性舱。用于确定治疗反应和肿瘤复发的现有临床工具主要受灵敏度的限制，主要是因为它们间接测量骨髓内部的肿瘤负担，并且未能捕获与mm相关的斑块多颗肿瘤渗透。微粒（MPS）是0.1至1.0μm的膜囊泡，其含有其始发细胞的细胞含量。 MPS是功能介质，传达孕激素，预种植剂，序列和促炎信息，建立细胞间交叉谈话并绕过许多病理中的直接细胞 - 细胞接触的需要。在该研究中，使用流式细胞术分析从De identified mm患者（n = 64）和正常受试者（n = 18）的血浆细胞衍生的Mps（CD138 +）。使用扫描电子显微镜进一步分析MP的形态和尺寸。我们的研究表明MM患者中MPS的系统签名证明。我们观察到MM的MP水平与肿瘤负担相对应的mm显着升高。我们提供了MM患者外周血在个性化MM临床监测中存在潜在应用的MPS存在的第一个证据。

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