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首页> 外文期刊>New Zealand Journal of Crop and Horticultural Science >Orthogonal test design for optimising the culture medium for in vitro pollen germination of feijoa (Acca sellowiana cv. Unique)
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Orthogonal test design for optimising the culture medium for in vitro pollen germination of feijoa (Acca sellowiana cv. Unique)

机译:优化Feijoa的体外花粉萌发的优化培养基的正交试验设计(<斜斜体> Acca Sellowiana-/ Italic> Cv。独特)

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ABSTRACT Feijoa suffers from high rates of flower and fruit abortion, and widely varying fruit characteristics, which are directly related to pollination problems. However, efficient pollination mostly depends on pollen vigour. Thus, methods to improve pollen vigour have become an important focus for both breeders and growers. An orthogonal design method (L_(9)[3]~(4)) was used to compare the effectiveness of key factors (e.g. concentrations of sucrose, H_(3)BO_(3), CaCl_(2) and GA_(3)) to determine pollen vigour, and to optimise the culture medium for in vitro pollen germination. The four factors that influenced pollen germination in order of significance are as follows: GA_(3)?&?CaCl_(2)?&?H_(3)BO_(3)?&?sucrose. These variables influenced pollen tube growth in the following order: CaCl_(2)?&?H_(3)BO_(3)?&?sucrose?&?GA_(3). The optimal culture medium for promoting pollen vigour was 10% sucrose, 1.95?×?10~(–3)?mol/L H_(3)BO_(3), 4?×?10~(–4)?mol/L CaCl_(2) and 80?mg/L GA_(3).
机译:摘要Feijoa患有高的花和果实流产率,以及广泛不同的果实特征,与授粉问题直接相关。然而,有效的授粉主要取决于花粉活力。因此,改善花粉活力的方法已成为育种者和种植者的重要焦点。正交设计方法(L_(9)[3]〜(4))用于比较关键因子的有效性(例如蔗糖的浓度,H_(3)BO_(3),CACL_(2)和GA_(3) )确定花粉活力,并优化体外花粉萌发的培养基。一种影响花粉萌发的四个因素如下:Ga_(3)?&Δcacl_(2)?&Δh_(3)bo_(3)?&蔗糖。这些变量以下列顺序影响了花粉管的增长:CaCl_(2)?&Δh_(3)Bo_(3)?&蔗糖α&α,Ga_(3)。用于促进花粉活力的最佳培养基是10%蔗糖,1.95?×10〜(3)ΔBo_(3),4?×10〜(-4)?mol / l CaCl_(2)和80?Mg / L Ga_(3)。

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