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首页> 外文期刊>Revista rvore >CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION
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CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

机译：Neem（Azadirachta indama A. Juss）的克隆繁殖通过直接和间接的体外再生

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The study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.
机译：该研究用Neem（Azadirachta Indica A. Juss）的射击尖端进行了进行，以确定可行的再生过程。从补充有BAP和培养基的DKW培养基中交替培养的Neem胚胎获得射击尖端。初始拍摄发育受宫内节子存在的影响。从体外茎碱中切除的基底愈伤组织也呈现了有机源性潜力。在一些情况下，从每种种子获得的植物线呈现不同的特征。在体外观察到的最常见的特征在茎碱上是愈伤组织形成。然而，最稀有的特征是干扰愈伤组织形成和叶片衰老。再生芽尖端进一步脱培养并根植于补充有IBA的培养基上，以便可以获得完整的植物。生根植物被移植到温室并成功地驯化。从愈伤组织和射击尖端传播之间观察到内省植物之间没有显着差异。

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