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首页> 外文期刊>Rice >Genomic and transcriptomic analysis reveal molecular basis of salinity tolerance in a novel strong salt-tolerant rice landrace Changmaogu
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Genomic and transcriptomic analysis reveal molecular basis of salinity tolerance in a novel strong salt-tolerant rice landrace Changmaogu

机译：基因组和转录组分析显示了一种新型强盐耐稻兰德克服巨大矿物质耐受性的分子基础

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Abstract BackgroundSalt stress is an important factor that limits rice yield. We identified a novel, strongly salt tolerant rice landrace called Changmaogu (CMG) collected from a coastal beach of Zhanjiang, Guangdong Province, China. The salt tolerance of CMG was much better than that of the international recognized salt tolerant rice cultivar Pokkali in the germination and seedling stages.ResultsTo understand the molecular basis of salt tolerance in CMG, we performed BSA-seq for two extreme bulks derived from the cross between CMG and a cultivar sensitive to salt, Zhefu802. Transcriptomic sequencing was conducted for CMG at the germination and young seedling stages. Six candidate regions for salt tolerance were mapped on Chromosome 1 by BSA-seq using the extreme populations. Based on the polymorphisms identified between both parents, we detected 32 genes containing nonsynonymous coding single nucleotide polymorphisms (SNPs) and frameshift mutations in the open reading frame (ORF) regions. With transcriptomic sequencing, we detected a large number of differentially expressed genes (DEGs) at the germination and seedling stages under salt stress. KEGG analysis indicated two of 69 DEGs shared at the germination and seedling stages were significantly enriched in the pathway of carotenoid biosynthesis. Of the 169 overlapping DEGs among three sample points at the seedling stage, 13 and six DEGs were clustered into the pathways of ABA signal transduction and carotenoid biosynthesis, respectively. Of the 32 genes carrying sequence variation, only OsPP2C8 (Os01g0656200) was differentially expressed in the young seedling stage under salt stress and also showed sequence polymorphism in the ORFs between CMG and Zhefu802.Conclusion OsPP2C8 was identified as the target candidate gene for salinity tolerance in the seedling stage. This provides an important genetic resource for the breeding of novel salt tolerant rice cultivars.
机译：摘要Backgroundsalt ressure是限制稻米产量的重要因素。我们确定了一种名为Changmaogu（CMG）的新型耐盐性耐麸稻兰，从中国广东省广东省湛江沿海地区收集。 CMG的耐盐性远远优于萌发和幼苗阶段的国际公认的耐盐水稻品种Pokkali的耐药性。培养型耐盐性在CMG中的分子基础，我们对来自十字架的两个极端块进行了BSA-SEQ在CMG和盐的栽培品种之间，ZEFU802。在发芽和幼苗阶段进行转录组测序。使用极端群体，BSA-SEQ映射到染色体1的含抗盐耐受的六个候选地区。基于父母两者之间鉴定的多态性，我们检测到32个基因含有非同义一体编码单核苷酸多态性（SNP）和开放阅读框（ORF）区域的帧突变突变。通过转录组测序，我们在盐胁迫下检测到萌发和幼苗阶段的大量差异表达基因（DEGS）。 Kegg分析表明，在胡萝卜素生物合成的途径中显着富集了在发芽和幼苗阶段共享的69只共享中的两个。在幼苗阶段的三个样品中的169个重叠的液体中，分别分别聚集到ABA信号转导和类胡萝卜素生物合成的途径中聚集13和六次。在携带序列变异的32个基因中，在盐胁迫下仅在幼苗阶段仅差异表达OSPP2C8（OS01G0656200），并且还在CMG和ZHEFU802之间的ORF中显示序列多态性。结论OSPP2C8被鉴定为盐度耐受性的目标候选基因幼苗阶段。这为新型耐盐水稻品种的育种提供了重要的遗传资源。

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