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首页> 外文期刊>Open Journal of Immunology >Sensitivity of a Rapid Mix Test with Combined Synthetic Antigens Derived from Mycobacterium Leprae PGL-1 for Diagnosis and Surveillance of Leprosy
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Sensitivity of a Rapid Mix Test with Combined Synthetic Antigens Derived from Mycobacterium Leprae PGL-1 for Diagnosis and Surveillance of Leprosy

机译：快速混合试验的敏感性与源自分枝杆菌的合成抗原-1-1衍生出诊断和监测麻风病

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Introduction: In the Americas, Brazil contributes 91.63% of the total cases and the state of Pará still has high endemia for leprosy. Objective: To analyze the performance of a rapid test for the diagnosis and epidemiological surveillance of leprosy in endemic areas. Methods: The sample consisted of 70 MB multibacillary leprosy (MB) patients, 63 paucibacillary (PB) patients, and 80 intradomiciliary consanguineous contacts (ICSCO) of patients. A rapid test with a 15-minute reading was applied using two prototypes: prototype 1, double test with trisaccharide antigen (NT-P-BSA) at 1a. line (83.2 ng/test) and disaccharide antigen (ND-O-BSA) at 2a. (83.2 ng/test), both with a flow of 0.08 μL/mm with a 10 μC membrane, anti-IgM conjugate with a flow of 0.040 μL/mm and a Tris-Triton and prototype 2 runner buffer with MIX antigen (trisaccharide + disaccharide) in the same concentrations and conditions of prototype 1. Results: The comparison of the MIX test positivity rate and the disaccharide or trisaccharide doublet test across all samples was statistically significant, demonstrating that the MIX test had higher seropositivity rates compared to the ND-O-BSA or NT-P-BSA. It was demonstrated that the MIX test showed a good performance, with 25.39% of the PB patients negative for the disaccharide and trisaccharide duplet test, but positive for MIX. Conclusions: These data suggest the potential for further optimizing the performance by adding other synthetic antigens to the MIX antigens.
机译：介绍：在美洲，巴西占总案件的91.63％，帕拉州仍然具有高罪名的麻风病。目的：分析流行区麻风病诊断和流行病学监测的快速试验的性能。方法：该样品由70 MB多元菌麻风病（MB）患者组成，63例患者63例患者，80例患有80例心脏阴性近亲接触（ICSCO）。使用两个原型涂覆15分钟的快速测试：原型1，在1A的三糖抗原（NT-P-BSA）进行双重试验。线（83.2ng /试验）和二糖抗原（ND-O-BSA）在2A。 （83.2ng / ist），均具有0.08μl/ mm的流动，10μc膜，抗IgM缀合物，流动，0.040μl/ mm，Tris-triton和具有混合抗原的原型2步道缓冲液（三糖+在相同的浓度和原型的浓度和原型的条件下的结果O-BSA或NT-P-BSA。结果表明，混合试验表现出良好的性能，25.39％的Pb患者对二糖和三糖双重试验负阴性，但混合阳性。结论：这些数据表明通过将其他合成抗原添加到混合物抗原来进一步优化性能的可能性。

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