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首页> 外文期刊>Open Journal of Urology >One-Step Multiplex PCR for Simultaneous Detection and Identification of Eight Medically Important &i&Candida&/i& Species
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One-Step Multiplex PCR for Simultaneous Detection and Identification of Eight Medically Important &i&Candida&/i& Species

机译：一步多重PCR，用于同时检测和鉴定八个医学上重要的＆ i＆念珠菌＆ / i＆物种

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摘要

Recently, the incidence of Candida infections has substantially increased.?Conventional identification methods for Candida species are technically difficult to conduct and cannot accurately distinguish each species. The purpose of the?present study was to design primers to identify and detect simultaneously eight medically important Candida species using one-step multiplex PCR.?PCR primers were designed based on partial sequences of intergenic spacer (IGS) and internal transcribed spacer (ITS) genes of eight medically important Candida species. These primers were able to distinguish each Candida species and did not display cross-reactivity with representative Candida species other than the eight Candida species. Moreover, our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.
机译：最近， Candida感染的发病率显着增加。念珠菌物种的核鉴定方法技术上难以进行，不能准确地区分每个物种。本研究的目的是设计引物，用于使用一步多重PCR的同时鉴定和检测同时八个医学上重要的念珠菌物种。基于基于代族间隔物（IGS）和内转录的间隔物的部分序列设计了PCR引物（ITS）八个医学上重要的念珠菌种类。这些引物能够区分每个念珠菌物种，并没有与八个

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