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Polarimetric multiphoton microscopy for the analysis of ocular structures

机译：Polarimetric Multiphoton显微镜用于分析眼结构

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This review article summarizes the main contributions of the PhD Thesis titled “Polarization andmultiphoton microsocopy for the analysis of ocular structures” carried out at the Laboratorio de ópticaof the University of Murcia. This work presents mathematical methods developed to both analyze andclassify the external or micrometric scale of type I collagen-based ocular tissues. On the one hand, atheoretical model has been developed to explore the polarization dependence of second harmonicgeneration signal. This allowed obtaining an analytical expression to calculate the internal ormolecular collagen organization. On the other hand, an experimental relationship between externaland internal collagen organization has been stablished. Finally, the Stokes-Mueller formalism has beenapplied to compute the polarization properties of type-I fibrillar collagen and to improve thevisualization of local structures. These methods have been proposed as a differential clinical tool forthe diagnosis of corneal keratoconus.
机译：该审查文章总结了穆尔西亚大学的Laboratoriodeópticaof的“极化和偏振和分析眼部结构分析”的博士论文的主要贡献。该工作提出了为分析的数学方法和Classify基于I型胶原蛋白的眼组织的外部或微米级。一方面，已经开发出无情模型来探索第二个次谐波信号的极化依赖性。这允许获得分析表达以计算内部或分子胶原组织。另一方面，已经稳定了OferyAnd内部胶原蛋白组织之间的实验关系。最后，Stokes-Mueller形式主义已经缺乏，以计算I型纤维蛋白的偏振性能，并改善局部结构的血清化。这些方法已被提出作为诊断角膜角膜核糖的差异临床工具。

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