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首页> 外文期刊>Molecular Metabolism >Skeletal muscle in healthy humans exhibits a day-night rhythm in lipid metabolism
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Skeletal muscle in healthy humans exhibits a day-night rhythm in lipid metabolism

机译：健康人体中的骨骼肌在脂质代谢中表现出日夜节奏

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Objective Human energy metabolism is under the regulation of the molecular circadian clock; we recently reported that mitochondrial respiration displays a day-night rhythm under study conditions that are similar to real life. Mitochondria are interconnected with lipid droplets, which are of importance in fuel utilization and play a role in muscle insulin sensitivity. Here, we investigated if skeletal muscle lipid content and composition also display day-night rhythmicity in healthy, lean volunteers. Methods Skeletal muscle biopsies were obtained from 12 healthy lean male volunteers every 5?h over a 24?h period. Volunteers were provided with standardized meals, and biopsies were taken 4.5?h after each last meal. Lipid droplet size and number were investigated by confocal microscopy. Additionally, the muscle lipidome was assessed using UPLC/HRMS-based semi-targeted lipidomics. Results Confocal microscopy revealed diurnal differences in intramyocellular lipid content ( P ?0.05) and lipid droplet size in oxidative type 1 muscle fibers ( P ?0.01). Lipidomics analysis revealed that 13% of all detected lipids displayed significant day-night rhythmicity. The most rhythmic lipid species were glycerophospholipids and diacylglycerols (DAG), with the latter being the largest fraction (50% of all rhythmic species). DAG levels showed a day-night pattern with a trough at 1 PM and a peak at 4 AM. Conclusions Using two distinct methods, our findings show that myocellular lipid content and whole muscle lipid composition vary across the day-night cycle under normal living conditions. In particular, day-night rhythmicity was present in over half of the DAG lipid species. Future studies are needed to investigate whether rhythmicity in DAG is functionally related to insulin sensitivity and how this might be altered in prediabetes.
机译：目标人能量代谢是在分子昼夜节点的调节下;我们最近报道，线粒体呼吸在类似于现实生活的研究条件下显示日夜节奏。线粒体与脂液滴相互联系，这些液滴在燃料利用方面具有重要性，并在肌肉胰岛素敏感性中发挥作用。在这里，我们调查了骨骼肌脂质含量和组合物也在健康的精益志愿者中显示日夜节奏性。方法骨骼肌活检每5μm24℃，每5°H，每5μm，每5°H。志愿者被提供有标准化的膳食，每次饭后都拍摄了4.5℃的活组织检查。通过共聚焦显微镜研究了脂质液滴尺寸和数量。另外，使用基于UPLC / HRMS的半靶向脂质学评估肌肉脂质体。结果共聚焦显微镜显示蛋白细胞脂质含量（P <0.05）和氧化型1肌纤维中的脂质液滴尺寸（P <0.01）。脂族分析显示，所有检测到的脂质的13％显示出大量的昼夜节律。最有节奏的脂质种类是甘油磷脂和二酰基甘油（DAG），后者是最大的级分（占所有节奏物种的50％）。 DAG水平显示日夜模式，下午1点下槽，凌晨4点峰值。我们的研究结果结论，我们的研究结果表明，在正常的生活条件下，肌细胞脂质含量和整个肌肉脂质组合物在夜间循环中变化。特别是，昼夜节律性存在于超过一半的DAG脂质物种中。未来的研究需要调查DAG中的节律性是否与胰岛素敏感性有关，并且如何在Prediapetes中改变。

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