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首页> 外文期刊>ScientificWorldJournal >Both Freshly Prepared and Frozen-Stored Amniotic Membrane Cells Express the Complement Inhibitor CD59
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Both Freshly Prepared and Frozen-Stored Amniotic Membrane Cells Express the Complement Inhibitor CD59

机译：新鲜制备和冷冻储存的羊膜细胞均表达补体抑制剂CD59

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Amniotic membrane proved to be very effective tool in the treatment of a number of ocular surface diseases. The amniotic membrane, however, has to be stored before its transplantation onto the ocular surface followed by mandatory serologic control in order to exclude the transmission of certain viruses. Therefore it is most important to study if cryopreservation of the membrane affects cell surface expression of the molecules. We measured cell surface expression of CD59, a membrane-bound complement inhibitor on the cells of freshly prepared and cryopreserved amniotic membrane. Cells of amniotic membrane were separated mechanically. Epithelial and mesenchymal cells were identified by the intracellular expression of nanog and the cell surface ICAM1 positivity, respectively. Multicolor flow cytometric immunophenotyping was used for determination of the CD59 expression. CellQuest-Pro software program (Becton Dickinson) was used both for measurements and analysis. CD59-positive cells could be detected in all investigated samples and in all investigated cell types, although the expression level of CD59 differed. CD59 was expressed both on freshly prepared and frozen-stored samples. Higher level of CD59 was detected on ICAM1+ mesenchymal cells than on nanog+ epithelial cells. Our findings indicate that amniotic membranes maintain their complement inhibiting capacity after cryopreservation.
机译：羊膜被证明是非常有效的工具治疗多种眼部疾病。然而，羊膜必须在将其移植到眼表面上储存，然后是强制性的血清学对照，以排除某些病毒的透射。因此，如果膜的冷冻保存影响膜的冷冻保存影响分子的细胞表面表达是最重要的。我们测量CD59的细胞表面表达，膜结合的补体抑制剂对新制备的和冷冻保存的羊膜细胞上的膜结合的补体抑制剂。机械分离羊膜细胞。通过分别的细胞内表达和细胞表面ICAM1阳性的细胞内表达鉴定上皮和间充质细胞。多色流式细胞术免疫蛋白型用于测定CD59表达。 CellQuest-Pro软件程序（Becton Dickinson）用于测量和分析。在所有研究样品中可以检测到CD59阳性细胞，但在所有研究的细胞类型中，尽管CD59的表达水平不同。 CD59在新鲜制备和冷冻储存的样品上表达。在ICAM1 +间充质细胞上检测到比纳米+上皮细胞更高水平的CD59。我们的研究结果表明，羊膜在冷冻保存后保持其补充抑制能力。

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