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首页> 外文期刊>Silvae genetica >Characterization of EST-SSRs for European beech (Fagus sylvatica L.) and their transferability to Fagus orientalis Lipsky, Castanea dentata Bork., and Quercus rubra L.
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Characterization of EST-SSRs for European beech (Fagus sylvatica L.) and their transferability to Fagus orientalis Lipsky, Castanea dentata Bork., and Quercus rubra L.

机译：欧洲山毛榉（Fagus Sylvatica L.）的EST-SSRS表征及其对Fagus Orientalis Lipsky，Castanea Dentata Bork的可转移性。和Quercus Rubra L.

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Due to ongoing climate change, forests are exposed to changing environmental conditions, such as increasing temperatures and lower precipitation, to which trees have to adapt. Successful adaptation to changing and variable environments requires sufficient genetic variation within tree populations. Knowledge of the genetic variation of trees is therefore essential, as it provides information for the long-term conservation, stability and productivity of forests. The genetic variation of a species can be analysed with molecular markers. Despite growing genomic and genetic resources for European beech (Fagus sylvatica L.), which is one of the economically and ecologically most important forest tree species in Central Europe, the number of molecular markers for population genetic analyses is still limited. Therefore, the aim of the work is the development of new EST-SSR markers for this species. A total of 72 DNA samples of European beech from three widely separated regions in Germany were used to test 41 primers for variation and polymorphism, 35 of which were originally developed for American beech (Fagus grandifolia Ehrh.) and 6 for red oak (Quercus rubra L.). Fifteen of the primers were polymorphic, 13 monomorphic and 13 did not amplify. In addition, the transferability of the markers was successfully tested in the related species Castanea dentata Bork., Fagus orientalis Lipsky and Q. rubra. The EST-SSR markers tested in this study will be useful for future population genetic analyses and extend the set of available markers in European beech.
机译：由于持续的气候变化，森林暴露于改变的环境条件，例如越来越多的温度和降水量，树木必须适应的树木。成功适应改变和可变环境需要在树群中需要足够的遗传变异。因此，知识树木的遗传变异是必不可少的，因为它为森林的长期守恒，稳定性和生产力提供了信息。可以用分子标记分析物种的遗传变异。尽管欧洲山毛榉（Fagus Sylvatica L）产生了种族组织和遗传资源，但这是中欧经济和生态最重要的森林树种之一，但群体遗传分析的分子标志数仍然有限。因此，这项工作的目的是为此物种开发新的EST-SSR标记。德国三个广泛分离的地区共有72个欧洲山毛榉样品用于测试41个底漆，用于变异和多态性，其中35个原始为美国山毛榉（Fagus Grandifolia Ehrh。）和6个用于红橡树（Quercus Rubra） L.）。十五个引物是多态，13个单孔，13个未扩增。此外，标志物的可转移性在相关的物种Castanea Dentata Bork中成功进行了测试。，Fagus Orientalis Lipsky和Q. Rubra。本研究中测试的EST-SSR标记对于未来的群体遗传分析将有助于延长欧洲山毛榉的可用标志物。

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