...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Standards in Genomic Sciences >Complete genome sequence of the lignin-degrading bacterium Klebsiella sp. strain BRL6-2
【24h】

Complete genome sequence of the lignin-degrading bacterium Klebsiella sp. strain BRL6-2

机译：完整的木质素降解细菌Klebsiella sp的基因组序列。菌株BRL6-2

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated Klebsiella sp. strain BRL6-2 on minimal media with alkali lignin as the sole carbon source. This organism was isolated anaerobically from tropical forest soils collected from the Bisley watershed at the Ridge site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are characterized by cycles of iron oxidation and reduction. Genome sequencing was targeted because of its ability to grow on lignin anaerobically and lignocellulolytic activity via in vitro enzyme assays. The genome of Klebsiella sp. strain BRL6-2 is 5.80 Mbp with no detected plasmids, and includes a relatively small arsenal of genes encoding lignocellulolytic carbohydrate active enzymes. The genome revealed four putative peroxidases including glutathione and DyP-type peroxidases, and a complete protocatechuate pathway encoded in a single gene cluster. Physiological studies revealed Klebsiella sp. strain BRL6-2 to be relatively stress tolerant to high ionic strength conditions. It grows in increasing concentrations of ionic liquid (1-ethyl-3-methyl-imidazolium acetate) up to 73.44 mM and NaCl up to 1.5 M.
机译：努力发现能够降解的厌氧细菌，我们孤立克莱布拉SP。用碱木质素作为唯一碳源的菌株BRL6-2。该生物体从来自美国波多黎各的El Yunque National Foration的山地山脉的热带森林土壤中厌氧地分离出来的热带森林土壤，其中包括Luquillo长期生态研究站的一部分。在该网站上，土壤在氧化还原潜力中经历了强烈的波动，并以铁氧化和减少的循环为特征。基因组测序是靶向的，因为它能够通过体外酶测定在木质素厌氧和木质纤维素溶解活性上生长。 Klebsiella sp的基因组。菌株BRL6-2是5.80mbp，没有检测到的质粒，包括编码木质纤维素溶解碳水化合物活性酶的相对小的基因库。该基因组揭示了四种推定的过氧化物酶，包括谷胱甘肽和DYP型过氧化物酶，以及在单个基因簇中编码的完整的原始细胞途径。生理学研究揭示了Klebsiella sp。菌株BR16-2是对高离子强度条件的相对应激耐受性。它在增加离子液体（1-乙基-3-甲基 - 咪唑鎓）的浓度增加至73.44mm，NaCl高达1.5米的浓度增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号