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首页> 外文期刊>Pain research & management: the journal of the Canadian Pain Society = journal de la socie?te? canadienne pour le traitement de la douleur >Systematic Administration of B Vitamins Alleviates Diabetic Pain and Inhibits Associated Expression of P2X3 and TRPV1 in Dorsal Root Ganglion Neurons and Proinflammatory Cytokines in Spinal Cord in Rats
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Systematic Administration of B Vitamins Alleviates Diabetic Pain and Inhibits Associated Expression of P2X3 and TRPV1 in Dorsal Root Ganglion Neurons and Proinflammatory Cytokines in Spinal Cord in Rats

机译：B维生素的系统施用减轻了糖尿病疼痛，抑制了大鼠脊髓脊髓中P2X3和TRPV1的相关表达和脊髓促炎细胞因子

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Background . Treatment of diabetic neuropathic pain (DNP) continues to be a major challenge, and underlying mechanisms of DNP remain elusive. We investigated treatment effects of B vitamins on DPN- and DNP-associated alterations of neurochemical signaling in the nociceptive dorsal root ganglion (DRG) neurons and the spinal cord in rats. Methods . DNP was produced in male, adult, Sprague Dawley rats by single i.p. streptozotocin (STZ). Western blot analysis and immunohistochemistry were used to analyze protein expressions in DRG and ELISA to measure the proinflammatory cytokines in the spinal cord. Behaviorally expressed DNP was determined by measuring the sensitivity of hindpaw skin to mechanical and thermal stimulation. Results . There were 87.5% (77/88) rats which developed high blood glucose within 1-2 weeks following STZ injection. Of which, 70.13% ( n =?54/77) animals exhibited DNP manifested as mechanical allodynia and/or thermal hyperalgesia. Intraperitoneal administration of vitamins B1/B6/B12 (100/100/2?mg/kg, one or multiple doses) significantly attenuated DNP without affecting the blood glucose. Expressions of P2X3 and TRPV1 in CGRP-positive and IB4-positive DRG neurons as well as the interleukin-1 β , tumor necrosis factor- α , and nerve growth factor in the lumbar spinal cord were greatly increased in DNP rats. Such DNP-associated neurochemical alterations were also greatly suppressed by the B-vitamin treatment. Conclusions . B-vitamin treatment can greatly suppress chronic DNP and DNP-associated increased activities of P2X3 and TRPV1 in DRG and the spinal proinflammatory cytokines, which may contribute to the pathogenesis of DNP. Systematic administration of B vitamins can be a strategy for DNP management in clinic.
机译：背景 。糖尿病神经病疼痛（DNP）的治疗仍然是一个重大挑战，DNP的潜在机制仍然难以捉摸。我们调查了B维生素对伤害伤害性背根神经节（DRG）神经元和大鼠脊髓的DPN和DNP相关改变的治疗效果。方法 。 DNP在雄性，成人，Sprague Dawley大鼠中生产的单身I.P.链脲佐菌素（STZ）。 Western印迹分析和免疫组织化学用于分析DRG和ELISA中的蛋白质表达，测量脊髓中的促炎细胞因子。通过测量后爪皮肤与机械和热刺激的敏感度来确定行为表达的DNP。结果 。在STZ注射后1-2周内出现了87.5％（77/88）大鼠，在1-2周内开发出高血糖。其中，70.13％（n = 54/77）动物表现出DNP表现为机械异常性疼痛和/或热痛觉过敏。腹膜内施用维生素B1 / B6 / B12（100/100/2？Mg / kg，一种或多剂量）显着减弱DNP而不影响血糖。在DNP大鼠中，CGRP阳性和IB4阳性DRG神经元以及白细胞介素-1β，肿瘤坏死因子α和神经生长因子的表达在DNP大鼠中大大增加。通过B-维生素治疗也大大抑制了这种DNP相关的神经化学改变。结论。 B-维生素治疗可以大大抑制DRG和脊髓促炎细胞因子的P2X3和TRPV1的慢性DNP和DNP相关的活性，这可能有助于DNP的发病机制。系统施用B维生​​素可以是诊所DNP管理的策略。

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