...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pakistan journal of botany >Molecular cloning and expression of two genes encoding ACCase subunits of Camellia oleifera (Theaceae).
【24h】

Molecular cloning and expression of two genes encoding ACCase subunits of Camellia oleifera (Theaceae).

机译：两种基因编码山细胞（Theaceae）的分子克隆和表达。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The heteromeric acetyl coenzyme A carboxylase (ACCase) in plant seeds catalyzes the formation of malonyl-CoA from acetyl-CoA, which is the rate-limiting step in de novo fatty acid biosynthesis. In this study, we cloned biotin carboxylase (BC) and β-subunits of carboxyltransferase (β-CT) genes of ACCase subunits from Camellia oleifera , namely Co-accC and Co-accD , respectively. The full-length Co-accC (GeneBank accession no.FJ965288) was 1901 bp encoded 530 aa, and the coding regions of Co-accD was 1530 bp encoded 510 aa (accession no. FJ965289), which was in the 2574-bp fragments of rbcL, rbcL-accD intergenic spacer and a ccD . The comparison of genomic and cDNA sequences showed that they were all intronless. Structural analyses showed that their putative amino acid sequences shared high identity with those of other oil-plants, and that they possessed the ATP-binding site, biotin carboxylation site in the Co-accC protein, and zinc finger motif CX 2 CX 15 CX 2 C in the Co-accD protein. Moreover, transcript expressions of the two genes were carried out in cultivars as well as in tissues and fruit development stages of ‘Huashuo’, the former showed that their expression levels in cultivars were almost correlated with oil contentsof matured seed kernels, the higher expression levels, the higher oil content to some extent, suggesting that they could potentially be as molecular markers for selection of higher oil production cultivars. The latter revealed their expression rules in lipid synthesis and accumulation. Taken together, information from our study indicates that they may be significant in the fatty acid and lipid biosynthesis of seeds, thus be valuable for oil yield of C. oleifera .
机译：植物种子中的异聚乙酰辅酶A羧基酶（Accase）催化来自乙酰-CoA的丙二酰CoA的形成，这是Novo脂肪酸生物合成的速率限制步骤。在本研究中，我们克隆了甲基油碱基和共址的Accase亚基的羧甲基酶（β-CT）基因的生物素羧化酶（BC）和β-亚基。全长CO-ACCC（GeneBank登录号码FJ965288）是1901年BP编码530 AA，Co-ACCD的编码区是1530bp编码的510 AA（登录号FJ965289），其在2574-BP碎片中RBCL，RBCL-ACCD代谢间隔物和CCD。基因组和cDNA序列的比较显示它们都是无内部的。结构分析表明，其推定的氨基酸序列与其他石油植物的副型序列共享高度，并且它们具有共址蛋白的ATP结合位点，生物素羧化位点，以及锌指基象CX 2 CX 15 CX 15 CX 2 CX 2 CX 2 C在共址蛋白中。此外，两种基因的转录表达在品种中，以及“华沙”的组织和果实发育阶段，前者表明它们在品种中的表达水平与成熟的种子核的油含量几乎相关，表达水平更高，较高的油含量在一定程度上，表明它们可能是选择更高油生产品种的分子标记。后者揭示了脂质合成和积累中的表达规则。从我们的研究中获取的信息表明，它们在种子的脂肪酸和脂质生物合成中可能是显着的，因此对C. Oleifera的油产量有价值。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号