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首页> 外文期刊>Pakistan journal of botany >DE NOVO TRANSCRIPTOME SEQUENCEING AND COMPARATIVE ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN DRYOPERIS FRAGRANS UNDER TEMPERATURE STRESS
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DE NOVO TRANSCRIPTOME SEQUENCEING AND COMPARATIVE ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN DRYOPERIS FRAGRANS UNDER TEMPERATURE STRESS

机译：在温度胁迫下，DEDOV转录组序列序列和比较分析Dyentiberis Fragrans在温度胁迫下

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Dryopteris fragrans is a species of fern and contains flavonoids compounds with medicinal value. This study explainthe temperature stress impact flavonoids synthesis in D. fragrans tissue culture seedlings under the low temperature at 4oC,high temperature at 35oC and moderate temperature at 25oC. By using Illumina HiSeq 2000 sequencing, 80.9 million rawsequence reads were de novo assembled into 66,716 non-redundant unigenes. 38,486 unigenes (57.7%) were annotated fortheir function. 13,973 unigenes and 29,598 unigenes were allocated to gene ontology (GO) and clusters of orthologousgroup (COG), respectively. 18,989 sequences mapped to 118 Kyoto Encyclopedia of Genes and Genomes Pathway database(KEGG), 204 genes were involved in flavonoid biosynthesis, regulation and transport. 25,292 and 16,817 unigenes exhibitedmarked differential expression in response to temperature shifts of 25oC to 4oC and 25oC to 35oC, respectively. 4CL andCHS genes involved in flavonoid biosynthesis were tested and suggested that they were responsible for biosynthesis offlavonoids. This study provides the first published data to describe the D. fragrans transcriptome and should accelerateunderstanding of flavonoids biosynthesis, regulation and transport mechanisms. Since most unigenes described here weresuccessfully annotated, these results should facilitate future functional genomic understanding and research of D. fragrans.
机译：Dryophteris Fragrans是一种蕨类植物，含有具有药物价值的黄酮类化合物。本研究利用4℃，高温在45℃下的低温下的温度胁迫在D.FrAgans组织培养幼苗中的影响。通过使用Illumina Hiseq 2000测序，8090万Rawsequence Reads读成De Novo组装成66,716个非冗余的unigenes。 38,486 unigenes（57.7％）被提示出来。分别分配13,973个unigenes和29,598个unigenes分配给基因本体（GO）和直际群组（COG）簇。 18,9899个序列映射到118 kyoto的基因和基因组途径（Kegg），204个基因参与黄酮类生物合成，调控和运输。 25,292和16,817 unigenes响应于25℃至4℃和25oC的温度转变，分别呈现出差异表达。研究了参与黄酮类生物合成的4CL Andchs基因，并表明它们负责生物合成的离职物质。本研究提供了第一个公布的数据来描述D.Fragrans转录组，并应加速黄酮类生物合成，调节和运输机制。由于这里描述的大多数未经成本恶化地注释，这些结果应促进未来的功能基因组理解和对D.Fragrans的研究。

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