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首页> 外文期刊>Pakistan journal of botany >Evaluation of sodium alginate and calcium chloride on development of synthetic seeds
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Evaluation of sodium alginate and calcium chloride on development of synthetic seeds

机译：藻酸钠和氯化钙对合成种子发育的评价

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The present investigation highlighted the role of sodium alginate (Na-alginate) and calcium chloride (CaCl 2 ) treatments on encapsulation of sugarcane ( Saccaharum officinarum var. NSG-59) somatic embryos to produce synthetic seeds. Progress in the protocol for direct recovery of plants from man-made seeds under sterile conditions is of major importance. Although it is possible to produce a huge number of plants through tissue culture via embryogenesis or multiple shoot cultures, but their delivery is quite inconvenient. In vitro study pertaining to evaluate the efficient synthetic seeds formation from sugarcane somatic embryos was done in Plant Tissue Culture Lab, Department of Botany, University of Sargodha. Encapsulation of somatic embryos was achieved by using Na-alginate (as gelling agent) and CaCl 2 (as complexing agent) respectively through dropping method under sterile conditions (inside Laminar air flow cabinet). Different concentrations of Na-alginate (0.5%, 1%, 2%, 2.5%, 3%, 3.5%, and 4%) and CaCl 2 (50mM, 70mM, 90mM, 100mM, 150mM and 200mM) were applied in current process.The gelling matrix of 3% Na-alginate and complexing agent of 100mM CaCl 2 yielded firm, clear, isodiametric and compact beads formation. It was observed that duration of Na-alginate treatment on bead formation has no effect however, CaCl 2 treatment with 100mM Na-alginate for 15 minutes was more appropriate for isodiametric and compact beads formation. The synthetic seeds provide feasible handling, transport and large scale production of viable plants of sugarcane by direct sowing of synthetic seeds in the soil.
机译：本研究强调了藻酸钠（Na-藻酸盐）和氯化钙（CaCl 2）治疗甘蔗（Saccaharum OfficinArum Var。NSG-59）细胞胚胎产生合成种子的作用。在无菌条件下从人造种子直接恢复植物方案的进展具有重要意义。虽然可以通过胚胎发生或多次芽培养产生大量的植物，但它们的递送非常不方便。在Sargodha大学植物组织文化实验室中，在植物组织培养实验室中进行了评价甘蔗体细胞胚的有效合成种子的体外研究。通过在无菌条件下使用Na-藻酸盐（作为胶凝剂）和CaCl 2（作为络合剂）来实现体细胞胚的封装（作为胶凝剂的空气流动柜内）。在当前过程中，应用不同浓度的Na-藻酸盐（0.5％，1％，2％，2.5％，3％，3.5％和4％）和CaCl 2（50mm，70mm，90mm，100mm，150mm，200mm） 。胶凝基质3％Na-藻酸盐和100mm CaCl 2的络合剂产生了坚固，透明，异摩擦和紧凑型珠粒。观察到珠子形成对藻酸盐处理的持续时间没有效果，但加入100mM Na-藻酸盐的CaCl 2处理15分钟更适合于异摩擦和紧凑珠粒形成。通过直接播种土壤中的合成种子，合成种子通过直接播种土壤中的合成种子提供可行的处理，运输和大规模生产甘蔗的可行植物。

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