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首页> 外文期刊>Pakistan journal of botany >Successful callogenesis from leaf and petiole of Bergenia ciliata (Haw.) Sternb and antibacterial activity of callus extracts
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Successful callogenesis from leaf and petiole of Bergenia ciliata (Haw.) Sternb and antibacterial activity of callus extracts

机译：来自叶片和叶柄的成功的胼ciliata（haw。）睾丸提取物的叶片和抗菌活性

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Medicinal plants have been used for centuries to prevent and treat different health disorders. Bergenia ciliata is native to Himalayas and is used traditionally as an antipyretic, an astringent, diuretic and for dissolving kidney and bladder stones. Due to the medicinal value of B. ciliata, it has been over-exploited leading to be classified an an endangered species. The current study was designed to see B. ciliata response to tissue culturing specifically to callus formation and antibacterial activity of callus extracts. Callus inducing media was supplemented with 2, 4-D and Kinetin. Visible signs of callus formation on cut edges of explants were observed after 13 and 14 days of culturing and leaf showed 95% while petiole depicted 88% callus induction, respectively. Highest dry weight of callus was obtained from petioles (0.61 mg), which was 12% higher than that of dry weight of callus produced by leaf explants on same medium. The ethanolic extract of both petiole and petiole-derived callus demonstrated promising antibacterial activity against six pathogenic bacterial strains. The results suggested that callus from valuable medicinal plants can provide a way to produce essential metabolites and help in the conservation of the natural population of endangered plants. In future, evaluating biochemical composition of callus and plants extracts will enable us to find new dimensions to get maximum benefits from in-vitro technique.
机译：药用植物已被用于几个世纪以预防和治疗不同的健康障碍。 Bergenia Ciliata原产于喜马拉雅山，传统上使用作为一种解热，涩，利尿剂和溶解肾脏和膀胱结石。由于B.Ciliata的药物价值，已经过度剥削导致归类为濒危物种。目前的研究旨在参见特异性促进愈伤组织培养和愈伤组织提取物的愈伤组织培养的CILIATA反应。愈伤组织诱导培养基补充有2,4-D和Kinetin。在13至14天的培养物和叶片显示出95％的同时，观察到外植体的切割边缘的愈伤组织愈伤组织形成迹象，同时叶柄分别描绘了88％的愈伤组织诱导。从叶柄（0.61mg）获得最热的愈伤组织（0.61mg），比相同培养基的叶子外植体产生的愈伤组织的干重12％。叶柄和叶柄衍生的愈伤组织的乙醇提取物证明了针对六种病原细菌菌株的有前途的抗菌活性。结果表明，来自宝贵的药用植物的愈伤组织可以提供一种方法来生产基本的代谢物，并帮助保护濒危植物的天然群体。将来，评估愈伤组织和植物提取物的生化组成将使我们能够找到新的尺寸以获得最大的效果。

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