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首页> 外文期刊>Pakistan journal of botany >Response of antioxidants and lipid peroxidation to exogenous application of alpha-tocopherol in sunflower ( Helianthus annuus L.) under salt stress
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Response of antioxidants and lipid peroxidation to exogenous application of alpha-tocopherol in sunflower ( Helianthus annuus L.) under salt stress

机译:抗氧化剂与脂质过氧化在盐胁迫下向日葵(Helianthus Anuus L.)外源应用的响应

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摘要

Alpha-tocopherol is an antioxidant mainly responsible for detoxification of reactive oxygen species produced during prolonged abiotic stresses as reported in the previous studies. A pot experiment was carried out to study the response of antioxidants and lipid peroxidation to alpha-tocopherol seed soaking treatment in saline conditions. Seeds of sunflower cvs. FH-[572 and 621] were immersed for 16 hours in four alpha-tocopherol levels, [0 (dist. H 2 O), 100, 200, 300] mg L -1 . Thirty five days after seed sowing plants were irrigated with 0 m M and 120 m M (two levels) of salt (NaCl) till final harvest. Leaves were sampled 61 days after seed sowing for biochemical analyses and yield at maturity. Salt stress (120 m M NaCl) caused significant reduction in shoot and root dry weight, total phenolic contents and superoxide dismutase activity and had non-significant effect on all yield related parameters. Salt stress showed substantial increase in the activity of enzymatic antioxidants such as catalase, peroxidase and glutathione reductase. Seed soaking with alpha-tocopherol significantly increased shoot and root dry weight, 100 achene weight and total achene weight plant -1 , catalase and peroxidase activity, total phenolic content, ascorbic acid and showed non-significant effect on leaf total soluble proteins and hydrogen peroxide. Alpha-tocopherol pre-sowing seed treatment caused significant reduction in lipid peroxidation. Cultivar FH-621 showed higher salt tolerance and alpha tocopherol 200 and 300 mg L -1 levels effectively increased its yield and dry matter production respectively under salt stress.
机译:α-生育酚是一种抗氧化剂,主要负责在先前研究中报道的延长非生物胁迫期间产生的反应性氧物种的解毒。进行了罐实验以研究抗氧化剂和脂质过氧化对盐条件下α-生育酚种子浸渍处理的响应。向日葵CV的种子。将FH-[572和621]浸入四个α-生育酚水平中16小时,[0(邻离H 2 O),100,200,300] Mg L -1。将种子播种植物灌溉盐(两级)的盐(NaCl)灌溉三十五天,直至最终收获。种子播种以进行生化分析并在成熟时产生叶片后61天取样。盐胁迫(120 m米NaCl)导致芽和根系干重,总酚类含量和超氧化物歧化酶活性的显着降低,对所有收率相关参数产生了非显着影响。盐应激显示酶促抗氧化剂如过氧化氢酶,过氧化物酶和谷胱甘肽还原酶的活性增加。用α-生育酚浸泡的种子浸泡芽和根系干重,100种成酸的重量和总瘦果植物 - 1,过氧化氢酶和过氧化物酶活性,总酚类含量,抗坏血酸,并对叶片总可溶性蛋白和过氧化氢显示出非显着影响。 α-生育酚预播种种子处理导致脂质过氧化的显着降低。品种FH-621显示出较高的耐盐性和α生育酚200和300mg L -1水平分别在盐胁迫下有效增加其产率和干物质产生。

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