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首页> 外文期刊>Pakistan journal of botany >DNA barcoding and molecular systematics of selected species of family Acanthaceae
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DNA barcoding and molecular systematics of selected species of family Acanthaceae

机译：DNA条形码和分子系统的选定品种刺槐

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In the present study the phylogeny of the various selected genera of Acanthaceae was investigated based on macro-morphological characters, chloroplast DNA (cp-DNA) genes rbc L, mat K and intergenic spacer trnH-psbA sequences. About 34 macro-morphological characters of taxonomic importance were selected from the different floras of the world which were used to construct a phylogeny based on morphology. For morphological analysis selected species belonging to five different genera of Acanthaceae including Ruellia, Justicia, Barleria , Dicliptera and Strobilanthes were studied . The phylogenetic tree was constructed using Multivariate Statistical Package (MVSP 3.1) by the method of UPGMA (Unweighted Pair Group Method Analysis). Our results showed that Ruellia brittoniana seemed to be the closest relative of Justicia and Barleria species based on morphological analysis . The plastid gene regions ribulose-1,5-bisphosphatecarboxylase/oxygenase ( rbc L), maturase kinase ( mat K) and trnH – psbA intergenic spacer region were selected for taxonomic study on molecular level. These genes rbc L (1400 bp), mat K (800 bp) and trnH – psbA spacer (400 bp) were sequenced and the sequence data was aligned and used for the phylogenetic tree in combination with the other sequences retrieved from GenBank, National Centre of Biotechnology Information (NCBI). Molecular data based on these mat K, rbc L and trnH –psbA sequences and the BLAST result shows that genera Barleria, Strobilanthes and Ruellia are monophyletic but genus Justicia is non-monophyletic. Our results conclude that rbc L is more effective barcode; while combination of mat K and trnH – psbA can be used for identification and phylogenetic study of plants.
机译：在本研究中，研究了基于宏观形态特征，叶绿体DNA（CP-DNA）基因RBC L，MAT K和代际间隔TrnH-PSBA序列研究了各种所选蜡的蜡的系统发育。从世界各地的不同植物群中选择了大约34种分类物理性的宏观形态特征，其用于基于形态学构建文学发生。对于形态学分析，研究了属于五种不同的Acanthaceae的物种，包括Reellia，Justicia，Barleria，Dicliptera和Strobilanthes。通过UPGMA方法（未加权对组方法分析）使用多元统计包（MVSP 3.1）构建系统发育树。我们的研究结果表明，基于形态学分析，Ruellia Brittoniana似乎是Justicia和Barleria物种的最接近。体液基因区核糖糖 - 1,5-双磷酸羧羧酶/氧酶（RBC1），升水酶激酶（MAT K）和TRNH - PSBA代族间隔区分类学研究分类学研究。这些基因RBC L（1400bp），垫K（800bp）和TrnH - psba间隔物（400bp）被测序，并将序列数据与来自Genbank，国家中心检索的其他序列组合对齐并用于系统发育树生物技术信息（NCBI）。基于这些垫K，RBC L和TRNH -PSBA序列和BLAST结果的分子数据表明，Genera Barleria，Strobilanthes和Reellia是单味般的，但贾斯基亚属是非单象的。我们的结果得出结论，RBC L更有效条形码;虽然Mat K和TrnH - PSBA的组合可用于植物的鉴定和系统发育研究。

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