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首页> 外文期刊>Pharmaceutics >Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!
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Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!

机译：非病毒体外基因递送：现在是时候设置酒吧！

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摘要

Transfection by means of non-viral gene delivery vectors is the cornerstone of modern gene delivery. Despite the resources poured into the development of ever more effective transfectants, improvement is still slow and limited. Of note, the performance of any gene delivery vector in vitro is strictly dependent on several experimental conditions specific to each laboratory. The lack of standard tests has thus largely contributed to the flood of inconsistent data underpinning the reproducibility crisis. A way researchers seek to address this issue is by gauging the effectiveness of newly synthesized gene delivery vectors with respect to benchmarks of seemingly well-known behavior. However, the performance of such reference molecules is also affected by the testing conditions. This survey points to non-standardized transfection settings and limited information on variables deemed relevant in this context as the major cause of such misalignments. This review provides a catalog of conditions optimized for the gold standard and internal reference, 25 kDa polyethyleneimine, that can be profitably replicated across studies for the sake of comparison. Overall, we wish to pave the way for the implementation of standardized protocols in order to make the evaluation of the effectiveness of transfectants as unbiased as possible.
机译：通过非病毒基因递送载体转染是现代基因递送的基石。尽管资源倒入了更有效的转染剂的发展，但改善仍然缓慢而有限。值得注意的是，体外任何基因递送载体的性能严格依赖于每个实验室特异的若干实验条件。因此，缺乏标准测试在很大程度上促进了支撑重现性危机的不一致数据泛滥。研究人员寻求解决这个问题的方式是通过衡量新综合的基因递送载体的有效性以及看似众所周知的行为的基准。然而，这种参考分子的性能也受到测试条件的影响。本调查指出了非标准化的转染设置，并有关在此背景下认为相关的变量的有限信息作为此类未对准的主要原因。本综述提供了针对黄金标准和内部参考，25 kda聚乙烯亚胺优化的条件目录，以便在跨学习中获得有利可图。总体而言，我们希望为实施标准化方案的实施方式，以便评估转染剂的有效性尽可能不偏不倚。

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