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A sugarcane mosaic virus?vector for gene expression in maize

机译：甘蔗马赛克病毒？玉米基因表达的载体

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Zea mays L. ssp. mays (maize) is an important crop plant as well as model system for genetics and plant biology. The ability to select among different virus-based platforms for transient gene silencing or protein expression experiments is expected to facilitate studies of gene function in maize and complement experiments with stable transgenes. Here, we describe the development of a sugarcane mosaic virus?(SCMV) vector for the purpose of protein expression in maize. An infectious SCMV cDNA clone was constructed, and heterologous genetic elements were placed between the protein 1 (P1) and helper component-proteinase (HC-Pro) cistrons in the SCMV genome. Recombinant SCMV clones engineered to express green fluorescent protein (GFP), β-glucuronidase (GUS), or bialaphos resistance (BAR) protein were introduced into sweet corn (Golden × Bantam) plants. Documentation of developmental time courses spanning maize growth from seedling to tasseling showed that the SCMV genome tolerates insertion of foreign sequences of at least 1,809 nucleotides at the P1/HC-Pro junction. Analysis of insert stability showed that the integrity of GFP and BAR coding sequences was maintained longer than that of the much larger GUS coding sequence. The SCMV isolate from which the expression vector is derived is able to infect several important maize inbred lines, suggesting that this SCMV vector has potential to be a valuable tool for gene functional analysis in a broad range of experimentally important maize genotypes.
机译：Zea mays L. SSP。梅斯（玉米）是一个重要的作物植物以及遗传和植物生物学的模型系统。预期在瞬时基因沉默或蛋白质表达实验中选择基于病毒的平台的能力，以促进玉米中基因功能的研究和稳定的转基因的补体实验。在这里，我们描述了甘蔗马赛克病毒的发展（SCMV）载体，用于玉米中的蛋白质表达。构建了一种传染式SCMV cDNA克隆，并在SCMV基因组中置于蛋白质1（P1）和辅助组分 - 蛋白酶（HC-Pro）传中心之间的异源遗传元件。将重组的SCMV克隆改造以表达绿色荧光蛋白（GFP），β-葡糖醛酸糖苷酶（GUS）或Bialaphos抵抗（Bar）蛋白被引入甜玉米（金×Bantam）植物中。从幼苗到流血的发育时间课程的文献表明，SCMV基因组在P1 / HC-Pro结处耐受至少1,809个核苷酸的外序列的插入。插入稳定性的分析表明，GFP和条形编码序列的完整性维持器长于更大的GUS编码序列的完整性。 ScMV分离衍生的SCMV分离物能够感染若干重要的玉米自交系，表明该SCMV载体具有在广泛的实验重要玉米基因型中的基因功能分析的有价值工具。

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