• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant direct. >Mobility and localization of the iron deficiency-induced transcription factor bHLH039 change in the presence of FIT
【24h】

Mobility and localization of the iron deficiency-induced transcription factor bHLH039 change in the presence of FIT

机译：铁缺乏诱导的转录因子BHLH039的流动性和定位在适应性存在下的变化

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Regulation of iron (Fe) acquisition and homeostasis is critical for plant survival. In Arabidopsis, Fe deficiency-induced bHLH039 forms a complex with the master regulator FIT and activates it to upregulate Fe acquisition genes. FIT is partitioned between cytoplasm and nucleus, whereby active FIT accumulates more in the nucleus than inactive FIT. At the same time, there is so far no information on the subcellular localization of bHLH039 protein and how it is controlled. We report here that the bHLH039 localization pattern changes depending on the presence of FIT in the cell. When expressed in cells lacking FIT, bHLH039 localizes predominantly in the cytoplasm, including cytoplasmic foci in close proximity to the plasma membrane. The presence of FIT enhances the mobility of bHLH039 and redirects the protein toward primarily nuclear localization, abolishing its accumulation in cytoplasmic foci. This FIT-dependent change in localization of bHLH039 found in transient fluorescent protein expression experiments was confirmed in both leaves and roots of Arabidopsis transgenic plants, stably expressing hemagglutinin-tagged bHLH039 in wild-type or fit mutant background. This posttranslational mechanism for intracellular partitioning of Fe-responsive transcription factors suggests a signaling cascade that translates Fe sensing at the plasma membrane to nuclear accumulation of the transcriptional regulators.? 2019 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.
机译：铁（Fe）收购和稳态对植物存活至关重要。在拟南芥中，Fe缺乏诱导的BHLH039与主调节剂合适的复合物形成并激活它以上调Fe采集基因。适合在细胞质和核之间分配，由此活性拟合在核中累积比不活性合身更多。与此同时，迄今为止没有关于BHLH039蛋白的亚细胞定位的信息以及如何控制它。我们在此报告，BHLH039本地化模式根据细胞中的适应性而变化。当在缺乏拟合的细胞中表达时，BHLH039主要在细胞质中定位，包括紧邻质膜附近的细胞质焦灶。拟合的存在增强了BHLH039的迁移率，并将蛋白质重定向到主要核局部，从而消除其在细胞质焦点中的积累。在亚缔植物转基因植物的叶片和根中确认了瞬时荧光蛋白表达实验中发现的BHLH039的本地化变化，在野生型或适合突变体背景中稳定地表达血凝素标记的BHLHO39。这种用于细胞内分配Fe响应转录因子的后期性机制表明，信号级联，其在质膜上转化Fe感测到转录调节剂的核积累。 2019年的作者。植物直接发表美国植物生物学家和实验生物学协会和约翰瓦里和儿子有限公司

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号