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首页> 外文期刊>Plant Omics >The role of sucrose metabolizing proteins in hyperosmotic stress tolerance in sweet sorghum (Sorghum bicolor L. Moench)
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The role of sucrose metabolizing proteins in hyperosmotic stress tolerance in sweet sorghum (Sorghum bicolor L. Moench)

机译：蔗糖代谢蛋白在甜心高粱中血管胁迫耐受性的作用（高粱双子L. moench）

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Sugar accumulation is a common metabolic response under drought stress conditions. However, studies on the underlying molecular mechanisms of sugar accumulation under stress remain restricted. This study explores the role of sucrose metabolizing proteins in conferring tolerance to drought-induced hyperosmotic stress, and ultimately osmotic adjustment in sorghum. The effect of stress on sugar content, enzyme activity and gene expression was investigated. Sweet sorghum varieties (ICSV213 and ICSB338) differing in levels of drought tolerance were subjected to a 10-day water deficit period. Brix plant sap analysis indicated elevated total soluble sugar levels under water stress conditions in both varieties with ICSV213 demonstrating a higher brix content than ICSB338. HPLC analysis gave a decrease in sucrose levels and an increase in glucose and fructose concentrations in both varieties with ICSV213 demonstrating higher hexose levels. Enzyme activity levels of invertase, sucrose phosphate synthase and sucrose synthase were found to increase under stress in both varieties with ICSV213 invertase displaying the highest activity when compared to other sucrose metabolizing enzymes. Transcriptional expression of invertase and sucrose phosphate synthase (SPS) genes was significantly up regulated in ICSV213 under stressed conditions, whereas sucrose synthase (Susy) levels remained low in both varieties. Data obtained for sorghum variety ICSV213 points towards a relationship between hyperosmotic stress tolerance and the accumulation of solutes and sucrose metabolism proteins. Consequently variety ICSV213 may therefore be an excellent target for future development of hyperosmotic stress tolerant sweet sorghum.
机译：糖积累是干旱胁迫条件下的常见代谢反应。然而，对压力下糖积聚的潜在分子机制的研究仍然受到限制。该研究探讨了蔗糖代谢蛋白在赋予促进诱导的高骨肉胁迫的耐受性，并最终在高粱中进行渗透调整。研究了应力对糖含量，酶活性和基因表达的影响。在耐旱耐受性水平不同的甜高粱品种（ICSV213和ICSB338）受到10天的水资源赤字期。 Brix植物SAP分析表明，ICSV213两种品种的水胁迫条件下的总可溶性糖水平升高，证明了比ICSB338更高的Brix含量。 HPLC分析对蔗糖水平降低，葡萄糖和果糖浓度的增加，含有ICSV213，展示较高的己糖水平。在与其他蔗糖代谢酶相比时，发现酶活性的转化酶，蔗糖磷酸合酶和蔗糖合酶和蔗糖合成酶在两种变化中的胁迫下增加，其含有ICSV213转化酶。在胁迫条件下，在ICSV213中显着调节转化酶和蔗糖磷酸盐合酶（SPS）基因的转录表达，而蔗糖合酶（SUSY）水平在两种品种中仍然低。高粱品种ICSV213获得的数据朝向高骨折应力耐受性与溶质和蔗糖代谢蛋白的积累之间的关系。因此，品种ICSV213可以是过度肤质耐受甜高粱的未来发展的优秀目标。

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