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T-DNA-genome junctions form early after infection and are influenced by the chromatin state of the host genome

机译：感染后早期形成T-DNA-基因组结，受到宿主基因组的染色质状态的影响

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Agrobacterium tumefaciens mediated T-DNA integration is a common tool for plant genome manipulation. However, there is controversy regarding whether T-DNA integration is biased towards genes or randomly distributed throughout the genome. In order to address this question, we performed high-throughput mapping of T-DNA-genome junctions obtained in the absence of selection at several time points after infection. T-DNA-genome junctions were detected as early as 6 hours post-infection. T-DNA distribution was apparently uniform throughout the chromosomes, yet local biases toward AT-rich motifs and T-DNA border sequence micro-homology were detected. Analysis of the epigenetic landscape of previously isolated sites of T-DNA integration in Kanamycin-selected transgenic plants showed an association with extremely low methylation and nucleosome occupancy. Conversely, non-selected junctions from this study showed no correlation with methylation and had chromatin marks, such as high nucleosome occupancy and high H3K27me3, that correspond to three-dimensional-interacting heterochromatin islands embedded within euchromatin. Such structures may play a role in capturing and silencing invading T-DNA.
机译：农杆杆菌突然介导的T-DNA集成是植物基因组操纵的常见工具。然而，关于T-DNA积分是否朝向基因偏向或随机分布在整个基因组中，存在争议。为了解决这个问题，我们在感染后几个时间点在没有选择的情况下获得的T-DNA基因组结的高通量映射。早在感染后6小时检测T-DNA-基因组结。在整个染色体中显然是均匀的T-DNA分布，但检测到富含富有的基序和T-DNA边界序列微观同源的局部偏差。在卡那霉素 - 选择的转基因植物中先前分离的T-DNA集成位点的表观遗传景观的分析显示了与极低甲基化和核心占用的关联。相反，本研究的未选择的交界处表现出与甲基化的相关性，并且具有染色质标记，例如高核小体占用和高H3K27ME3，其对应于嵌入在Euchromatin内的三维相互作用的异铬脲岛。这种结构可以在捕获和沉默侵袭T-DNA中发挥作用。

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