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首页> 外文期刊>PLoS Genetics >FLS2 is a CDK-like kinase that directly binds IFT70 and is required for proper ciliary disassembly in Chlamydomonas
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FLS2 is a CDK-like kinase that directly binds IFT70 and is required for proper ciliary disassembly in Chlamydomonas

机译：FLS2是一种类似CDK样激酶，其直接结合IFT70，并且需要在<斜体>衣原体中适当的纤毛拆卸

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Intraflagellar transport (IFT) is required for ciliary assembly and maintenance. While disruption of IFT may trigger ciliary disassembly, we show here that IFT mediated transport of a CDK-like kinase ensures proper ciliary disassembly. Mutations in flagellar shortening 2 ( FLS2 ), encoding a CDK-like kinase, lead to retardation of cilia resorption and delay of cell cycle progression. Stimulation for ciliary disassembly induces gradual dephosphorylation of FLS2 accompanied with gradual inactivation. Loss of FLS2 or its kinase activity induces early onset of kinesin13 phosphorylation in cilia. FLS2 is predominantly localized in the cell body, however, it is transported to cilia upon induction of ciliary disassembly. FLS2 directly interacts with IFT70 and loss of this interaction inhibits its ciliary transport, leading to dysregulation of kinesin13 phosphorylation and retardation of ciliary disassembly. Thus, this work demonstrates that IFT plays active roles in controlling proper ciliary disassembly by transporting a protein kinase to cilia to regulate a microtubule depolymerizer.
机译：睫状体组件和维护需要肠道颗粒运输（IFT）。虽然IFT的破坏可能会引发睫状体脱索，但我们在此显示IFT介导的CDK样激酶的转运确保了适当的纤毛拆卸。鞭毛缩短2（FLS2）的突变，编码CDK样激酶，导致纤毛的吸收延迟和细胞周期进展的延迟。对睫状体拆卸的刺激诱导伴随逐渐失活的FLS2的逐渐去磷酸化。 FLS2的丧失或其激酶活性诱导纤毛中的Kinesin13磷酸化早期发作。 FLS2主要是在细胞体内定位的，然而，在诱导睫状体拆卸时它被运输到纤毛。 FLS2直接与IFT70相互作用，并且这种相互作用的丧失抑制其睫状体运输，导致Kinesin13磷酸化的失调和纤毛拆卸的延迟。因此，这项工作表明，IFT通过将蛋白激酶传送给Cilia来调节微管解聚有机化剂，IFT在控制适当的睫状体拆卸方面发挥活性作用。

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