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首页> 外文期刊>PLoS Genetics >Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons
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Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons

机译：在<斜视>果蝇感觉神经元中的树突修剪需​​要Rab11激活。

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摘要

Neuronal pruning is a commonly observed phenomenon for the developing nervous systems to ensure precise wiring of neural circuits. The function of Ik2 kinase and its downstream mediator, Spindle-F (Spn-F), are essential for dendrite pruning of Drosophila sensory neurons during development. However, little is known about how Ik2/Spn-F signaling is transduced in neurons and ultimately results in dendrite pruning. Our genetic analyses and rescue experiments demonstrated that the small GTPase Rab11, especially the active GTP-bound form, is required for dendrite pruning. We also found that Rab11 shows genetic interactions with spn-F and ik2 on pruning. Live imaging of single neurons and antibody staining reveal normal Ik2 kinase activation in Rab11 mutant neurons, suggesting that Rab11 could have a functional connection downstream of and/or parallel to the Ik2 kinase signaling. Moreover, we provide biochemical evidence that both the Ik2 kinase activity and the formation of Ik2/Spn-F/Rab11 complexes are central to promote Rab11 activation in cells. Together, our studies reveal that a critical role of Ik2/Spn-F signaling in neuronal pruning is to promote Rab11 activation, which is crucial for dendrite pruning in neurons.
机译：神经元修剪是显影神经系统的通常观察到的现象，以确保神经电路的精确布线。 IK2激酶及其下游介体，Spindle-F（SPN-F）的功能对于发育过程中果蝇感官神经元的枝晶修剪至关重要。然而，关于IK2 / SPN-F信号如何在神经元转导的并且最终导致树突修剪的毫无疑问。我们的遗传分析和救援实验表明，枝晶修剪需要小GTP酶Rab11，尤其是活性GTP结合形式。我们还发现RAB11显示了与SPN-F和IK2进行修剪的遗传相互作用。单一神经元和抗体染色的实时成像显示RAB11突变神经元中的正常IK2激酶活化，表明RAB11可以具有下游和/或平行于IK2激酶信号传导的功能连接。此外，我们提供了生物化学证据，即IK2激酶活性和IK2 / SPN-F / RAB11复合物的形成是促进细胞中的RAB11活化的中心。我们的研究揭示了IK2 / SPN-F信号在神经元修剪中的关键作用是促进RAB11活化，这对于神经元中的树突修剪至关重要。

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