...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>PLOS Neglected Tropical Diseases >Characterization of Phlebotomus papatasi Peritrophins, and the Role of PpPer1 in Leishmania major Survival in its Natural Vector
【24h】

Characterization of Phlebotomus papatasi Peritrophins, and the Role of PpPer1 in Leishmania major Survival in its Natural Vector

机译：PPPER1在天然载体中PPPER1在Leishmania主要存活中的作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The peritrophic matrix (PM) plays a key role in compartmentalization of the blood meal and as barrier to pathogens in many disease vectors. To establish an infection in sand flies, Leishmania must escape from the endoperitrophic space to prevent excretion with remnants of the blood meal digestion. In spite of the role played regarding Leishmania survival, little is known about sand fly PM molecular components and structural organization. We characterized three peritrophins (PpPer1, PpPer2, and PpPer3) from Phlebotomus papatasi. PpPer1 and PpPer2 display, respectively, four and one chitin-binding domains (CBDs). PpPer3 on the other hand has two CBDs, one mucin-like domain, and a putative domain with hallmarks of a CBD, but with changes in key amino acids. Temporal and spatial expression analyses show that PpPer1 is expressed specifically in the female midgut after blood feeding. PpPer2 and PpPer3 mRNAs were constitutively expressed in midgut and hindgut, with PpPer3 also being expressed in Malpighian tubules. PpPer2 was the only gene expressed in developmental stages. Interestingly, PpPer1 and PpPer3 expression are regulated by Le. major infection. Recombinant PpPer1, PpPer2 and PpPer3 were obtained and shown to display similar biochemical profiles as the native; we also show that PpPer1 and PpPer2 are able to bind chitin. Knockdown of PpPer1 led to a 44% reduction in protein, which in spite of producing an effect on the percentage of infected sand flies, resulted in a 39% increase of parasite load at 48 h. Our data suggest that PpPer1 is a component for the P. papatasi PM and likely involved in the PM role as barrier against Le. major infection.
机译：脱脂基质（PM）在血粕的舱室化和许多疾病载体中的病原体的障碍起到关键作用。为了在沙滩中建立感染，LeishMania必须逃离内骨渗透空间，以防止排泄与血液膳食消化的残余物。尽管有关于Leishmania生存的作用，但对于沙蝇PM分子组分和结构组织而言，很少。我们以斑斑瘤Papatasi特征为三种紫草林（PPPER1，PPPER2和PPPER3）。 PPPER1和PPPER2显示，分别为四个和一个丁蛋白结合结构域（CBD）。另一方面，PPPER3具有两种CBD，一种粘液状域，以及具有CBD的标志的推定域，但随着关键的氨基酸的变化。时间和空间表达分析表明，PPPER1在血液喂养后的雌性中肠中特异性地表达。 PPPER2和PPPER3 mRNA在中肠和后肠中组成型表达，PPPER3也在Malpighian小管中表达。 PPPER2是发展阶段中唯一表达的基因。有趣的是，PPPER1和PPPER3表达式由LE调节。主要感染。获得重组PPPER1，PPPER2和PPPER3并显示为原生物的生物化学型材;我们还表明PPPER1和PPPER2能够粘合依托粉。 PPPER1的敲低导致蛋白质的44％降低，这仍然不会对感染砂蝇的百分比产生影响，导致寄生虫载荷增加39％。我们的数据表明，PPPER1是P.Papatasi PM的组成部分，并且可能参与PM作为对LE屏障的障碍。主要感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号