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首页> 外文期刊>PLOS Neglected Tropical Diseases >Plasmodium vivax transcriptional profiling of low input cryopreserved isolates through the intraerythrocytic development cycle
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Plasmodium vivax transcriptional profiling of low input cryopreserved isolates through the intraerythrocytic development cycle

机译：通过卵细胞的开发循环进行低输入冷冻保存分离物的疟原虫转录分析

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Plasmodium vivax is the most prevalent malaria-causing parasite species outside of Sub-Saharan Africa and has many unique and poorly understood biological characteristics that make it particularly challenging to study and combat. Transcriptomic profiling of P. vivax under various conditions has the potential to unlock new experimental abilities and aid in elucidating biology and the development of clinical interventions. However, a lack of a robust in vitro culture system for this parasite has restricted transcriptomic studies to researchers with timely access to fresh human isolates from clinics, which often are in resource-poor settings, as well as nearby, well-equipped laboratories for sample processing. This study aimed to gain insight into the differential survival of P. vivax in various culture media from the parasites transcriptional signature in each media. By implementing low-input RNA library preparation strategies, this study obtains robust transcriptomic data at various parasite development stages and in different culture conditions from just 1000 FACS-purified, P. vivax-infected erythrocytes from viable cryopreserved patient isolates. With these data, we find culture media has little effect on transcriptional profile, we characterize invasion ligand expression across intraerythrocytic development and between clinical isolates, and we define the transcriptome of sexual, transmissible stages of the P. vivax parasite. These results highlight the establishment and utility of a powerful platform for studying the transcriptomic biology of this particularly challenging parasite.
机译：疟原虫疟原虫是撒哈拉以外地区以外的最普遍疟疾的疟疾物种，具有许多独特且知之甚少理解的生物学特征，使其特别具有挑战性地研究和战斗。在各种条件下，P.Vivax的转录组分析有可能解开新的实验能力和帮助阐明生物学和临床干预的发展。然而，对于该寄生虫的体外培养系统缺乏稳健的体外培养系统对研究人员来说，对来自诊所的新鲜人类分离物的研究人员来说，这通常是资源差的环境，以及附近的样品的附近的实验室加工。本研究旨在深入了解来自每种介质中的寄生虫转录签名的各种培养基中P.Vivax的差异存活。通过实施低输入RNA文库制备策略，本研究在各种寄生虫发育阶段和不同的培养条件下从可行的冷冻保存患者分离株中获得了寄生虫发育阶段的鲁棒转录组数据和不同的培养条件。通过这些数据，我们发现文化媒体对转录概况几乎没有影响，我们在细胞内肾癌症和临床分离株之间表征侵袭配体表达，并且我们定义了P.Vivax寄生虫的性透射阶段的转录组。这些结果突出了一个强大平台的建立和效用，用于研究这种特别具有挑战性的寄生虫的转录组生物学。

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