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首页> 外文期刊>Revista Brasileira de Ciência Avícola >Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens
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Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens

机译：含有1.25-二羟基胆胆碱对含有甘露糖浆果实鸡骨矿化和机械和组织性能的影响

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An experiment was conducted to investigate the effects of hydroalcoholic extract of Withania coagulans (WC) fruit and 1.25-dihydroxycholecalciferol (1.25-(OH) 2 D 3 ) on bone mineralization, mechanical and histological properties of male broiler chickens at 21 and 42 d of age. A total of six hundred male day-old Ross 308 broiler chickens were randomly distributed according to a completely randomized experimental design in a 2??3??2 factorial arrangement with 12 treatments of five replicates of 10 birds each. Treatments consisted of two basal diets (positive control with adequate Ca level and negative control with 30% less Ca), three levels of WC (0, 100, or 200 mg/kg diet), and two levels of 1.25-(OH) 2 D 3 (0 or 0.5 ?μg/kg diet). Birds were housed in floor pens. The diets were fed ad libitum from one to 42 days of age. On day 21 and 42, one bird per replicate was sacrificed and its tibiae were removed. Both Ca and P retention increased when dietary Ca level was reduced (p<0.001). The addition of 200 mg WC/kg to positive control diet increased Ca retention (p<0.01). Except for tibia diameter, no significant main effects of experimental treatments were observed on tibia physical characteristics or on bone mineralization. The diet with 30% Ca reduction decreased tibia diameter at 42 days of age (p<0.05). The dietary addition of 1.25-(OH) 2 D 3 increased tibia fracture energy, width of tibia mineralized zone, and serum Ca at 42 days of age (p<0.05). At 21 days of age, supplementation of 100 mg WC/kg increased cortical thickness (p<0.05). At 42 days of age, supplementation of 100 mg WC/kg increased tibia shear force (p<0.05) and fracture energy (p<0.01). The results of this experiment showed that supplementation of 100 mg/kg hydroalcoholic extract of WC fruit increased tibia cortical thickness, shear force, and fracture energy.
机译：进行了一个实验，以研究含有ania凝壳（WC）果实和1.25-二羟基胆总管（1.25-（OH）2 d 3）对骨矿化，男性肉鸡鸡的机械和组织学特性在21和42 d中的影响的实验年龄。共有六百名男性日龄罗斯308肉鸡鸡根据2 ?? 3的完全随机的实验设计随机分布，其中2个阶段排列，每次有12个鸟类的12种经济分析。治疗包括两个基础饮食（具有足够的Ca水平和阴性对照的阳性对照，30％减去CA），三种水平的WC（0,100或200mg / kg饮食），以及两个水平的1.25-（OH）2 D 3（0或0.5？μg/ kg饮食）。鸟类被安置在地板上。饮食从一次到42天内喂养自由。在第21和42天，处死每重复的一只鸟类，并将其胫骨移除。当膳食CA水平降低时，Ca和P保留都会增加（P <0.001）。向阳性对照饮食添加200mg Wc / kg增加了Ca保留（P <0.01）。除胫骨直径外，在胫骨物理特征或骨矿化上没有观察到实验治疗的显着主要影响。增量30％的饮食减少胫骨直径42天（P <0.05）。膳食添加1.25-（OH）2 d 3增加胫骨骨折能量，胫骨裂缝宽度，42天，血清CA（P <0.05）。在21天，补充100mg WC / kg的皮质厚度增加（P <0.05）。在42天时，补充100mg WC / kg增加胫骨剪切力（P <0.05）和骨折能量（P <0.01）。该实验的结果表明，补充了WC果汁的100mg / kg水醇提取物增加了胫骨皮质厚度，剪切力和断裂能。

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