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首页> 外文期刊>Revista Brasileira de Ciência Avícola >Establishment of a Pathogenicity Index for One-day-old Broilers to Pasteurella multocida Strains Isolated from Clinical Cases in Poultry and Swine
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Establishment of a Pathogenicity Index for One-day-old Broilers to Pasteurella multocida Strains Isolated from Clinical Cases in Poultry and Swine

机译：为单日肉鸡的致病性指数建立患有尿料和猪的临床病例中分离的巴斯特氏菌菌株

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Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 10 6 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening.
机译：虽然VieCeurella Multocada是呼吸道微生物群的成员，但在某些情况下，它是一种疾病的主要试剂，例如Fowl Cholera（Fc），导致经济损失显着。实验性接种可以用于评估菌株的致病性，但结果通常是主观的，并且对该试剂的发病机制的知识仍然有限。本研究的目的是通过制定标准指数来建立用于分类P. Multocida致病性的新方法。选择从FC病例和猪中分离出呼吸问题的菌株。在10天旧的肉鸡中接种每种菌株的一百毫升细菌培养物的每种菌株。接种后的死亡率，死亡时间（TD）和六个宏观病变的存在在接种后七天（DPI）的时间内评价。计算每只鸟（IPI），范围为0至10的致病性指数。收集肝脏和心脏片段以重新孤立细菌。从存活的鸟类收集血液，进行ELISA测试以检测特定抗体。致病性指数的中位数，病变的数量和细菌再辨比的速率显着不同（P <0.05）在分离物的起源中（P <0.05）。本研究中开发的致病性指数允许分类巴斯氏藻菌致病性，并且可以是目前用于筛选的致病性模型的替代品。

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