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首页> 外文期刊>Proceedings of the Japan Academy, Series B. Physical and Biological Sciences >Electron spectroscopic imaging (ESI) of cobalt ions responsible for the blockade of synaptic transmission and excitability of muscle cells in frog neuromuscular preparations
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Electron spectroscopic imaging (ESI) of cobalt ions responsible for the blockade of synaptic transmission and excitability of muscle cells in frog neuromuscular preparations

机译：钴离子的电子光谱成像（ESI）负责血液肌肉细胞突触突触突触肌肉细胞的抗突触神经肌肉制剂

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During electrical stimulation of the frog neuromuscular preparation in Ringer solution, CaCl2 (1.8mM) was replaced by equimolar CoCl2 (Co-Ringer solution). Muscular contraction due to the motor nerve stimulation was blocked completely within 2min, while muscular contraction due to direct muscle stimulation was reduced but not completely blocked. The tissue was fixed with 2.5% glutaraldehyde prepared in Co-Ringer solution. After rapid wash in Ringer solution, the tissue was transfered into an aqueous solution containing 10mM K3Fe(CN)6 (precipitating agent of Co2+) and 90mM NaCl. In conventional transmission electron microscopy, large precipitates were observed close to both sides of the presynaptic membrane, outside of the sarcolemma in subsynaptic and extrasynaptic area, on the myofibrils and close to both sides of the membrane of the sarcoplasmic reticulum in the vicinity of the T-tubules. It is presumed that the sites where the precipitates were localized correspond to the voltage dependent calcium channels of the presynaptic membrane, sarcoplasmic membrane and membrane of sarcoplasmic reticulum. The precipitates found on the myofibrils are considerd as Co2+ which penetrated the endplate channels and directed towards the sarcoplasmic reticulum. To our knowledge, the present electron microscopic observation of Co2+ precipitatedwith potassium ferricyanide is the first attempt in the cobalt cytochemistry.
机译：在ringer溶液中的青蛙神经肌肉制剂的电刺激期间，CaCl 2 （1.8mm）被等摩尔COCl 2 （共林溶液）代替。由于运动神经刺激引起的肌肉收缩完全在2min内完全封闭，而引起的肌肉刺激引起的肌肉收缩减少但未完全阻塞。用在共林氏溶液中制备的2.5％戊二醛固定组织。在Ringer溶液中快速洗涤后，将组织转移到含有10mm K 3 Fe（CN） 6 （CO 2 +的沉淀剂的水溶液中sup>）和90mm nacl。在传统的透射电子显微镜下，观察到突触前膜的两侧，突触膜的两侧靠近抗腹膜和额外区域，在肌纤维上，靠近T的膜的膜的两侧靠近T. -tubules。假设沉淀物局部化的位点对应于突触前膜，肌肉膜和肌浆网的膜的电压依赖性钙通道。在肌原纤维上发现的沉淀物被认为是CO 2 + / sup>，其穿透端板通道并向肌淋巴瘤引导。据我们所知，CO 2 + 沉淀的本电子显微镜观察沉淀为铁氰化钾是钴细胞化学的第一次尝试。

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