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首页> 外文期刊>Ukrainian Biochemical Journal >Indexes of nitric oxide system in experimental antiphospholipid syndrome
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Indexes of nitric oxide system in experimental antiphospholipid syndrome

机译：实验性抗磷脂综合征的一氧化氮系统指标

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Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of antibodies to negatively charged membrane phospholipids (aPL). Endothelial dysfunction is one of the most dangerous APS manifestations followed by thrombosis, placental insufficiency and often foetal death due to circulatory disorders in placenta blood vessels. It is established that synthesis and bioavailability of nitric oxide (NO) in the endothelium are impaired at APS, but the role of NO system in pregnancy failure at this pathology remains ambiguous. The aim of this research was to estimate the indexes of the nitric oxide system in animals with an experimental antiphospholipid syndrome before pregnancy and on the 18supth/sup day of pregnancy, without treatment and under treatment with nitric oxide synthesis modulators (L-arginine and aminoguanidine). In the blood serum and liver of the BALB/c mice with experimental APS, the content of eNOS and iNOSsup–/sup by ELISA and the level of NOsub2/subsup–/sup and NOsub3/subsup–/sup with the use of Gris reagent were determined before pregnancy and on the 18th day of pregnancy. The data obtained indicate the relative inefficient NO production by eNOS and NO hyperproduction by iNOS in the blood serum and liver of mice in the pathogenesis of experimental APS. Thus, in mice with APS before pregnancy and on the 18supth/sup day of the pregnancy, the eNOS content and NOsub2/subsup–/sup level were decreased while the iNOS content and NOsub3/subsup–/sup level were increased compared to the indexes in the control animal group. L-arginine administration to the animals with APS at the follow-up periods resulted in an increased eNOS content and NOsub2/subsup–/sup, NOsub3/subsup–/sup levels in blood serum and liver with the simultaneous decrease in iNOS content in the liver as compared to indexes in untreated mice with APS. The combined use of L-arginine and selective iNOS inhibitor aminoguanidine caused a significant increase in eNOS content and a decrease in iNOS content followed by normalization of NOsub2/subsup–/sup and NOsub3/subsup–/sup levels in blood and liver of mice with experimental APS before pregnancy and on the 18th day of pregnancy compared to untreated mice with APS.
机译：抗磷脂综合征（APS）是一种自身免疫性疾病，其特征在于抗体对带负电荷的膜磷脂（APL）的存在。内皮功能障碍是最危险的APS表现之一之一，随后由于胎盘血管中的循环疾病而导致血栓形成，胎盘不足和胎儿死亡。建立在APS的内皮中一氧化氮（NO）的合成和生物利用度受损，但在该病理学中没有系统在妊娠期衰竭中的作用仍然存在含糊不清。本研究的目的是估计在妊娠前的实验性抗磷脂综合征和18个妊娠的18个 Th 日，无需治疗，并且用一氧化氮合成调节剂治疗，估计有一氧化氮系统的指数（L-精氨酸和氨基胍）。在具有实验AP的BALB / C小鼠的血清和肝脏中，ELISA的ENOS和INOS的含量和NO 2 - < / sup>并且在妊娠之前和怀孕的第18天之前测定使用GRIS试剂的 3 - 。所获得的数据表明，在实验AP的发病机制中，在血清中，在血清中的血清和小鼠的肝脏中没有生产的相对效率。因此，在妊娠前的APS的小鼠和18个 Th 日的妊娠中，eNOS含量和否 2 - 水平在与对照动物组中的指标相比，InOS含量和NO 3 - 水平增加。 L-精氨酸在随访期间用AP的动物施用，导致eNOS含量增加，没有 2 - ，no 3 <与未处理的小鼠的指标相比，血清血清和肝脏在血清和肝脏中同时降低肝脏的含量，与APS未处理的小鼠的指标相比。 L-精氨酸和选择性InOS抑制剂氨基胍的组合使用引起eNOS含量的显着增加和InOS含量的减少，然后归一化NO 2 - 在妊娠前的实验APS和妊娠前的实验APS的血液和肝脏的3 - - 与未经治疗的小鼠有关的APS。

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