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首页> 外文期刊>Ukrainian Biochemical Journal >Sulfonamides influence on the activity of thylakoid ATPase isolated from spinach chloroplasts
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Sulfonamides influence on the activity of thylakoid ATPase isolated from spinach chloroplasts

机译：磺酰胺对菠菜叶绿体中分离的紫花状ATP酶的活性影响

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摘要

The effect of two water-soluble sulfonamides: aromatic 4-aminobenzenesulfonamide (SA) and aliphatic trifluoromethylsulfonamide (TFMSA) on the latent and stimulated ATPase activity of the catalytic part of the thylakoid ATP synthase isolated from spinach ( Spinacia oleracea L.) chloroplasts was studied. Three methods of ATPase activity stimulation were applied in the work: brief heating of the enzyme at 62 °С; incubation with 20% methanol; 25 mM sodium sulfite adding to the reaction medium. TFMSA effectively inhibited the ATPase activity of the enzyme at all types of activation. Isub50/sub (TFMSA) value for was found to be about 5?μM in the case of enzyme heat treatment and 1 μM in the case of enzyme activation with sulfite or methanol. SA did not affect ATPase activity at stimulation with sulfite and inhibited with Isub50/sub about 50 μM at stimulation by heating or methanol. No effect of SA on latent enzyme activity was observed, while TFMSA was shown to activate latent ATPase by about 6 times. The data obtained showed that both sulfonamide compounds, known as carbonic anhydrase inhibitors, are able to modify the activity of chloroplast ATPase.
机译：研究了两种水溶性磺胺酰胺（SA）和脂族三氟甲基磺酰胺（TFMSA）对从菠菜（Spinacia Oleracea L.）叶绿体的催化部分的潜伏和刺激的ATP酶活性（TFMSA）对抗刺痛（Spinacia Oleracea L.）叶绿体的潜伏和刺激的ATP酶活性的影响。在工作中应用三种ATP酶活性刺激方法：在62°С中短暂加热酶;孵育20％甲醇; 25mM亚硫酸钠加入反应介质。 TFMSA有效地抑制了所有类型的活化时酶的ATP酶活性。在酶热处理的情况下，发现I 50 （TFMSA）值在酶​​热处理的情况下为约5Ωμm，在酶活化亚硫酸盐或甲醇的情况下为1μm。 SA不影响硫酸盐刺激的ATP酶活性，并通过加热或甲醇在刺激时抑制I 50 约50μm。没有观察到SA对潜伏酶活性的影响，而TFMSA被证明以将潜伏的ATP酶激活约6次。得到的数据显示，磺酰胺化合物两种称为碳酸酐酶抑制剂，能够改变叶绿体ATP酶的活性。

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