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Overexpression of ANLN in lung adenocarcinoma is associated with metastasis

机译：肺腺癌中的Anln的过度表达与转移相关

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ANLN has been identified as an actin-binding protein and previous studies have suggested that ANLN is associated with actin cytoskeletal dynamics. Lung adenocarcinoma is a poor prognosis tumor. Metastasis is a very common complication and is regarded as the main cause for an unsatisfactory treatment outcome. Whether ANLN is involved in the metastasis of lung adenocarcinoma is unknown. We performed immunohistochemical staining and analyzed the correlation between the expression level and pathological parameters. We tested the migration and invasion of A549 and PC9 cell after interference with ANLN expression by Transwell and scratch wound healing assays. Protein expression of E-cadherin, vimentin and N-cadherin were detected using Western blotting and immunofluorescence staining. The same experiment was also tested after overexpression of ANLN. The metastasis of patients with high expression of ANLN was significantly more than that of patients with low expression of ANLN. In vitro, after interfering with ANLN expression, E-cadherin and vimentin expression were increased and N-cadherin expression was decreased in A549 and PC9 cells. Migration and invasion ability of A549 and PC9 cells were decreased,vice versa. Our study suggests that the expression of ANLN in lung adenocarcinoma is associated with metastasis of cancer cells. ANLN may be involved in the metastasis of lung adenocarcinoma by promoting epithelial mesenchymal transformation of tumor cells. ? 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.
机译：已被鉴定为肌动蛋白结合蛋白质和之前的研究表明ANLN与肌动蛋白细胞骨骼动态相关。肺腺癌是一种差的预后肿瘤。转移是一种非常常见的并发症，被认为是治疗结果不令人满意的主要原因。 ANLN是否参与肺腺癌转移是未知的。我们进行免疫组织化学染色并分析表达水平与病理参数之间的相关性。通过Transwell和划伤伤口愈合测定干扰ANLN表达后，测试A549和PC9细胞的迁移和侵袭。使用蛋白质印迹和免疫荧光染色检测E-Cadherin，Vimentin和N-Cadherin的蛋白质表达。在过表达ANLN后也测试了同样的实验。高表达ANLN患者的转移显着大于anln低表达的患者。体外，在干扰Anln表达后，e-cadherin和Vimentin表达增加，A549和PC9细胞中的N-钙粘蛋白表达降低。 A549和PC9细胞的迁移和侵袭能力降低，反之亦然。我们的研究表明，肺腺癌中的ANLN表达与癌细胞转移有关。通过促进肿瘤细胞的上皮间充质转化，ANLN可以参与肺腺癌转移。 ？ 2019年的作者。中国肺部肿瘤集团和约翰瓦里和儿子澳大利亚发表的胸癌

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