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首页> 外文期刊>Thoracic cancer. >Squalene epoxidase promotes the proliferation and metastasis of lung squamous cell carcinoma cells though extracellular signal-regulated kinase signaling
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Squalene epoxidase promotes the proliferation and metastasis of lung squamous cell carcinoma cells though extracellular signal-regulated kinase signaling

机译：Squalene环氧酶促进肺鳞状细胞癌细胞的增殖和转移虽然细胞外信号调节激酶信号传导

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The biological function of squalene epoxidase (SQLE), an important rate-limiting enzyme in downstream cholesterol synthesis, is to convert squalene to 2-3 oxacin squalene. The expression of SQLE in lung cancer is abnormal. We conducted this study to investigate the effect of SQLE expression on lung squamous cell carcinoma (SCC) proliferation, migration, and invasion and its role in extracellular signal-regulated kinase (ERK) signaling. Cell Counting Kit 8, wound healing, and Transwell assays; Western blotting; and quantitative real-time PCR were used to investigate the effect of SQLE in a lung SCC H520 cell line. Kaplan-Meier analysis was used to identify the prognostic significance of SQLE. Overexpression of SQLE promoted lung SCC cell proliferation, migration and invasion, whereas knockdown of SQLE expression showed the opposite effect. SQLE can interact with ERK to enhance its phosphorylation. SQLE may contribute to the pathogenesis of lung cancer by modulating ERK signaling. Further survival analysis indicated that high expression of SQLE indicated poor prognosis in lung SCC. Our study presents novel evidence of potential biomarkers or therapeutic targets for lung SCC therapy and prognosis. ? 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.
机译：角鲨烯环氧酶（SQLE）的生物学功能，下游胆固醇合成中的重要速率限制酶，是将Squalene转化为2-3个氧化甘油氏菌。 Sqle在肺癌中的表达是异常的。我们进行了该研究，探讨了SQLE表达对肺鳞状细胞癌（SCC）增殖，迁移和侵袭的影响及其在细胞外信号调节激酶（ERK）信号中的作用。细胞计数试剂盒8，伤口愈合和Transwell测定; Western Blotting;和定量实时PCR用于研究SQLE在肺SCC H520细胞系中的作用。 KAPLAN-MEIER分析用于识别SQLE的预后意义。 SQLE的过度表达促进了肺SCC细胞增殖，迁移和侵袭，而SQLE表达的敲低显示出相反的效果。 Sqle可以与ERK相互作用以增强其磷酸化。通过调节ERK信号传导，SQLE可能有助于肺癌的发病机制。进一步的生存分析表明，SQLE的高表达表明肺SCC预后差。我们的研究提出了潜在的生物标志物或治疗靶标的肺部SCC治疗和预后的新迹象。 ？ 2019年的作者。中国肺部肿瘤集团和约翰瓦里和儿子澳大利亚发表的胸癌

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