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首页> 外文期刊>Translational Neurodegeneration >Reprogrammed astrocytes display higher neurogenic competence, migration ability and cell death resistance than reprogrammed fibroblasts
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Reprogrammed astrocytes display higher neurogenic competence, migration ability and cell death resistance than reprogrammed fibroblasts

机译：重编程的星形胶质细胞显示出比重新编程的成纤维细胞更高的神经源性能力，迁移能力和细胞死亡率

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The direct reprogramming of somatic cells into induced neural progenitor cells (iNPCs) has been envisioned as a promising approach to overcome ethical and clinical issues of pluripotent stem cell transplantation. We previously reported that astrocyte-derived induced pluripotent stem cells (iPSCs) have more tendencies for neuronal differentiation than fibroblast-derived iPSCs. However, the differences of neurogenic potential between astrocyte-derived iNPCs (AiNPCs) and iNPCs from non-neural origins, such as fibroblast-derived iNPCs (FiNPCs), and the underlying mechanisms remain unclear. Our results suggested that AiNPCs exhibited higher differentiation efficiency, mobility and survival capacities, compared to FiNPCs. The whole transcriptome analysis revealed higher activities of TGFβ signaling in AiNPCs, versus FiNPCs, following a similar trend between astrocytes and fibroblasts. The higher neurogenic competence, migration ability, and cell death resistance of AiNPCs could be abrogated using TGFβ signaling inhibitor LY2157299. Hence, our study demonstrates the difference between iNPCs generated from neural and non-neural cells, together with the underlying mechanisms, which, provides valuable information for donor cell selection in the reprogramming approach.
机译：将体细胞的直接重新编程成诱导的神经祖细胞（INPC）被设想为克服多能干细胞移植的道德和临床问题的有希望的方法。我们之前报道，星形胶质细胞衍生的多能干细胞（IPSC）具有比成纤维细胞衍生的IPSC的神经元分化的趋势更多。然而，星形胶质细胞衍生的inpcs（AinpCs）和来自非神经源的inpcs之间的神经胶质潜力的差异，例如成纤维细胞衍生的inpcs（FinPcs），以及下面的机制仍然不清楚。我们的研究结果表明，与FinPC相比，Ainpcs表现出更高的分化效率，流动性和生存能力。在星形胶质细胞和成纤维细胞之间存在类似的趋势之后，整个转录组分析显示了Ainpcs中的TGFβ信号传导的活性，与FinPC相似。使用TGFβ信号抑制剂LY2157299可以消除αINPC的神经源性能力，迁移能力和细胞死亡抗性。因此，我们的研究表明，从神经和非神经细胞产生的inpc之间的差异与潜在的机制一起为在重编程方法中提供了供体细胞选择的有价值信息。

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