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首页> 外文期刊>Data in Brief >Mass spectrometry data from identification of host-defense related proteins using label-free quantitative proteomic analysis of milk whey from cows with Staphylococcus aureus subclinical mastitis
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Mass spectrometry data from identification of host-defense related proteins using label-free quantitative proteomic analysis of milk whey from cows with Staphylococcus aureus subclinical mastitis

机译:来自母牛奶的无标签定量蛋白质从牛乳清的鉴定中的质谱数据来自奶牛的牛奶,斜体>斜葡萄球菌亚临床乳腺炎

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This dataset is associated with our research article ‘Identification of host-defence related proteins using label-free quantitative proteomic analysis of milk whey from cows withStaphylococcus aureusSubclinical mastitis’ published in International Journal of Molecular Sciences. Milk samples were collected from cows suffering fromS. aureus-associated subclinical mastitis and the proteins abundance were identified in comparison with samples collected from the control cows using liquid chromatography-mass spectrometry (LC-MS)-based label free proteomics analysis. Following the MS measurements, the raw spectra were processed using MaxQuant-Andromeda software and the protein identification was carried out through a search against Uniprot FASTA files of theBos taurusreference proteome. Perseus software analysis was applied for computation of protein abundance. The raw file presented in this DiB was deposition at PRIDE repository in the PRIDE repository with the dataset identifierPXD007516.
机译:该数据集与我们的研究制品“鉴定宿主防御相关蛋白质的鉴定使用,使用牛奶乳清乳清乳清乳清乳清炎”在国际分子科学杂志上发表的标签定量蛋白质组分。从患有患有的奶牛收集牛奶样品。与使用液相色谱 - 质谱(LC-MS)的标记的游离蛋白质组学分析,与从对照牛收集的样品相比,鉴定了金黄色葡萄球菌相关的亚临床乳腺炎和蛋白质丰度。在MS测量后,使用MaxQuant-Andromeda软件处理原始光谱,通过搜索ZaurusReference蛋白质组的Uniprot Fasta档案进行蛋白质识别。 Perseus软件分析用于计算蛋白质丰度。在此DIB中呈现的原始文件在具有DataSet IdentifierPXD007516的Pride存储库中以普遍存储库沉积。

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