Data on the differentiation among <ce:italic>Leishmania</ce:italic> ( <ce:italic>Viannia</ce:italic>) spp., <ce:italic>Leishmania</ce:italic> ( <ce:italic>Leishmania</ce:italic>) <ce:italic>infantum</ce:italic> and <ce:italic>Leishmania</ce:italic> ( <ce:italic>Leishmania</ce:italic>) <ce:italic>amazonensis</ce:italic> in Brazilian clinical samples using real-time PCR

Aurora Diotallevi; Gloria Buffi; Marcello Ceccarelli; Herintha Coeto Neitzke-Abreu; Laisa Vieira Gnutzmann; Manoel Sebasti?o da Costa Lima Junior; Alice Di Domenico; Mauro De Santi; Mauro Magnani; Luca Galluzzi

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Data on the differentiation among Leishmania ( Viannia) spp., Leishmania ( Leishmania) infantum and Leishmania ( Leishmania) amazonensis in Brazilian clinical samples using real-time PCR

机译：关于 leishmania的差异数据（ viannia ）spp。， leishmania （莱山姆人） infantum 和 lealmania （ leishmania ）< CE：斜体> Amazonensis 在巴西临床样品中使用实时PCR

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This article contains the data regardingLeishmaniaspecies identification in human and canine clinical samples from a Brazilian region endemic forLeishmania(Viannia) spp.,Leishmania(Leishmania)infantumandLeishmania(Leishmania)amazonensis, using a previously developed approach involving two qPCR assays (qPCR-ML and qPCR-ama). The data are related to the article “Real-time PCR to differentiate amongLeishmania(Viannia) subgenus,Leishmania(Leishmania)infantumandLeishmania(Leishmania)amazonensis: application on Brazilian clinical samples” [1], and include also details of clinical evaluation/diagnosis of human patients and primer sequences used in the qPCR assays. TheLeishmaniaspecies has been determined in 27 canine samples and 11 human samples, exploiting HRM analysis of qPCR-ML and Cq values of qPCR-ML and qPCR-ama, as reported previously [2]. The qPCR data were in agreement with the species characterization obtained with other methods such as conventional species-specific PCR, ITS1 PCR-RFLP or DNA sequencing. Despite the limited number of clinical samples, these data are encouraging for a potential application in regions whereL.(Viannia) spp.,L. (L.)infantumandL. (L.)amazonensisare co-endemic.

机译：本文包含有关来自巴西地区的人和犬类临床样本中的物种和犬类临床样本的数据，来自巴西地区的人群流动的流动营地（viannia）SPP。 - 玛利斯）。这些数据与文章“实时PCR分化为区分中的（viannia）亚群，Leishmania（Leishmania）infantumandleishmania（Leishmania）Amazonensis：在巴西临床样本中的应用程序”[1]，并包括临床评估/诊断细节QPCR测定中使用的人类患者和引物序列。如先前报道的，QPCR-ML和QPCR-AMA的HRM分析已在27个犬样品和11人样品中确定了27个犬样本和11人样品，利用QPCR-ML和QPCR-AMA的HRM分析[2]。 QPCR数据与用其他方法获得的物种表征，例如常规物种特异性PCR，ITS1 PCR-RFLP或DNA测序。尽管临床样本数量有限，但这些数据令潜在的应用在地区林时。（viannnia）spp。，l。（L.）Infantumandl。（L.）Amazonensisare合作。

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《Data in Brief》 |2020年第2期|共8页
作者
Aurora Diotallevi; Gloria Buffi; Marcello Ceccarelli; Herintha Coeto Neitzke-Abreu; Laisa Vieira Gnutzmann; Manoel Sebasti?o da Costa Lima Junior; Alice Di Domenico; Mauro De Santi; Mauro Magnani; Luca Galluzzi;
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Leishmania infantumVianniaLeishmania amazonensisqPCRReal-time PCRHRM;

机译：Leishmania InfantumviannialeIshmaniaaiaaiaazonensisqpcrreal-time pcrhrm;

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Data on the differentiation among Leishmania ( Viannia) spp., Leishmania ( Leishmania) infantum and Leishmania ( Leishmania) amazonensis in Brazilian clinical samples using real-time PCR

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