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Ribbon Synapses and Hearing Impairment in Mice After in utero Sevoflurane Exposure

机译：在子宫七氟脲暴露后小鼠中的丝带突触和听力障碍

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Introduction: In utero, exposure to sevoflurane (a commonly used inhalation anesthetic) can lead to hearing impairment in offspring mice, but the underlying impairment mechanism is not known. Materials and Methods: Day-15 pregnant mice were treated with 2.5% sevoflurane for 2 h to investigate sevoflurane ototoxicity. Cochleae from offspring mice were harvested for hair-cell and ribbon-synapse assessments. Hearing in offspring mice was assessed at postnatal day 30 using an auditory brainstem-response (ABR) test. Cochlear-explant cultures from offspring mice were exposed to 2.5% sevoflurane for 6 h. Immediately after treatment, explants were assessed for hair-cell morphology, mitochondrial oxidative stress, and autophagy. Results: In utero, sevoflurane exposure impaired hearing in the offspring is demonstrated by a decrease in ABR wave I amplitudes, a marker for ribbon-synapse functionality. Sevoflurane exposure caused no obvious damage to hair cells, but cochlear ribbon synapses were reduced in postnatal day 15 offspring, and partially recovered by postnatal day 30. Sevoflurane treatment also increased mitochondrial reactive-oxygen species stress and decreased autophagy in the cochlear explants. Conclusion: These results suggest that oxidative stress and reduced autophagy may underly ribbon-synapse involvement in sevoflurane-induced hearing loss.
机译：介绍：在子宫内，暴露于七氟烷（常用的吸入麻醉剂）可以导致后代小鼠的听力障碍，但潜在的减值机制尚不清楚。材料和方法：用2.5％七氟醚处理Day-15妊娠小鼠2小时以研究七氟醚耳毒性。收获来自后代小鼠的耳果，用于毛细胞和带状康扑评估。使用听觉脑干 - 响应（ABR）测试在后期30日评估后代小鼠的听证。将后代小鼠的耳蜗外培养物暴露于2.5％七氟醚6小时。治疗后立即，评估外植体的毛细胞形态，线粒体氧化应激和自噬。结果：在子宫中，ABR波I幅度的减少，证明了在后代的七氟醚暴露在后代听力，用于带状突触功能的标记。七氟醚暴露对毛细胞没有明显损害，但在后期后的15天后，耳蜗丝带突触减少，并通过后期第30天部分回收。七氟醚处理也增加了线粒体反应性 - 氧物质应激并降低了耳蜗外的植物中的自噬。结论：这些结果表明，氧化应激和降低的自噬可能在七氟醚诱导的听力损失中具有显着的带状突触受累。

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