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首页> 外文期刊>Drug Design, Development and Therapy >Efficacy of Sub-Tenon Micro-Perfusion of Cyclophosphamide in Rabbits with Severe Ocular Inflammation
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Efficacy of Sub-Tenon Micro-Perfusion of Cyclophosphamide in Rabbits with Severe Ocular Inflammation

机译：具有严重眼炎症兔环磷酰胺的亚叛股微灌注的疗效

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Purpose: To explore the feasibility of cyclophosphamide (CP) via a sub-Tenon micro-perfusion system (SMS) in rabbits, and assess its therapeutic efficacy in severe ocular inflammation. Materials and Methods: Distribution and pharmacokinetics of CP were evaluated in vivo, and the concentrations of CP in plasma, vitreous humor, and retina/choroid were quantitated by ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) at different time points. After induction of severe experimental uveitis, rabbits were divided into three groups (n=8 in each): the SMS group, subconjunctival injection (SI) group, and control group. Clinical inflammatory score was assessed in rabbits. Electroretinography and histopathology were performed on post-treatment day 8. Statistical analyses were performed using Mann–Whitney and Kruskal–Wallis tests. P -value less than 0.05 was considered significant. Results: The concentrations of CP in vitreous humor and retina/choroid in the SMS group were significantly higher than that of the SI group at 3, 6, 10, and 24 hours ( P 0.05). The SMS group showed significantly less inflammation compared to the control group and SI group. Furthermore, the restoration of retinal structure and function were more obvious in the SMS group compared with conventional SI application. Conclusion: Sub-Tenon micro-perfusion of CP exhibited satisfied therapeutic efficacy in rabbits with severe ocular inflammation and may provide a promising alternative for controlling ocular inflammatory disease and immune-mediated ocular diseases.
机译：目的：探讨通过兔子胎对象微灌注系统（SMS）的环磷酰胺（CP）的可行性，并评估其严重眼炎症中的治疗效果。材料和方法：通过超高效液相色谱 - 串联质谱（UPLC-MS / MS）定量，在体内评估CP的分布和药代动力学，以及血浆，玻璃体幽默幽默和视网膜/脉络膜的浓度在不同的时间点。在诱导严重的实验葡萄膜炎后，将兔子分为三组（每次n = 8）：SMS组，亚细胞注射液（Si）组和对照组。在兔子中评估临床炎症评分。在治疗后第8天进行电化图和组织病理学。使用Mann-Whitney和Kruskal-Wallis测试进行统计分析。 P-value小于0.05被认为是显着的。结果：SMS组中玻璃体幽默和视网膜/脉络膜中CP的浓度明显高于3,6,10和24小时的Si组（P 0.05）。与对照组和SI组相比，SMS组显示出显着较少的炎症。此外，与传统Si应用相比，SMS组在SMS组中的恢复更明显。结论：CP的亚肾病微灌注表现出具有严重眼炎症的兔子的治疗效果，可提供对控制眼炎症和免疫介导的眼部疾病的有前途的替代方案。

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