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首页> 外文期刊>Drug Design, Development and Therapy >Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS
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Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS

机译：用HPLC-MS / MS微透过微透析对正常和脑缺血再灌注损伤血液和脑组织血液和脑组织的药代动力学分析

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Objective: The aim of our research was to analyze and compare the pharmacokinetics of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood and brain tissue of normal and cerebral ischemia-reperfusion injury rats by HPLC-MS/MS method. Methods: The blood and brain tissue samples of normal and middle cerebral artery occlusion (MCAO) rats were compared. The blood and brain tissue samples were collected by using microdialysis technique. The concentrations of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in blood and brain tissues were determined by the HPLC-MS/MS internal standard method. Results: Compared with the normal group, the model group after the administration of the Huangqi Guizhi Wuwu Decoction showed that Cmax blood, AUC0-t blood, and AUC0-inf blood of paeoniflorin were increased, CLblood, t1/2 brain, and Vbrain of paeoniflorin were decreased; Cmaxblood, AUC0-tblood, AUC0-infblood, and average residence time (MRTbrain) of calycosin-7-o-β-d-6-glucoside were decreased and the CLblood and Cmax brain of calycosin-7-o-β-d-6-glucoside were increased; Cmax blood of calycosin was decreased, Vblood and Vbrain of calycosin were increased; Cmax blood, AUC0-t blood, AUC0-inf blood, and MRTbrain of 6-gingerol were decreased, CLblood of 6-gingerol was increased. Conclusion: This method is simple, rapid, and sensitive. It is suitable for the pharmacokinetic study of Huangqi Guizhi Wuwu Decoction in the blood and brain tissue of rats. Cerebral ischemia-reperfusion injury increased the content of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood, affecting the clearance rate of paeoniflorin in the brain, the detention time of calycosin-7-o-β-d-6-glucoside and the 6-gingerol in the brain. In normal and cerebral ischemia-reperfusion rats, the content of paeoniflorin and 6-gingerol in the blood was higher than that in brain tissue, while the content of calycosin, calycosin-7-o-β-d-6-glucoside in the brain tissue was higher than that in blood, suggesting that calycosin and calycosin-7-o-β-d-6-glucoside have brain targeting properties.
机译：目的：我们的研究目的是分析和比较Paeoniflorin，Calycosin，Calycosin-7-O-β-D-6-葡萄糖苷和6-姜醇在正常和脑缺血再灌注的血液组织中的6-姜剂的药代动力学HPLC-MS / MS法损伤大鼠。方法：比较正常和中脑动脉闭塞（MCAO）大鼠的血液和脑组织样品。通过使用微透析技术收集血液和脑组织样品。通过HPLC-MS / MS内标法测定芍药蛋白，植物素，钙霉素-7-O-β-D-6-葡糖苷和6-姜醇的血液和脑组织中的6种。结果：与正常组相比，模型组在桂珠吐越后的给药显示，CMAX血液，AUC0-T血液和芍药素的AUC0-INF血液增加，CLBLOOD，T1 / 2脑，和vbrain Paeoniflorin被降低; Canaxblood，Auc0-Tblood，Auc0-Infblood和平均停留时间（MRTBrain）的钙霉素-7-o-β-D-6-葡糖苷的含量降低，Chlblood和Calycosin-7-O-β-D-的Cmax脑6-葡糖苷升高;降钙素的Cmax血液下降，vbloods of calycosin的vbrain; CMAX血液，AUC0-T血液，AUC0-INF血液和6-姜醇的MRTBRAIN减少，增加了6-姜醇的粘土。结论：这种方法简单，快速，敏感。它适用于大鼠血液和脑组织中黄芪桂珠的药代动力学研究。脑缺血再灌注损伤增加了芍药素，钙霉素，钙霉素-7-O-β-D-6-葡糖苷和血液6-姜醇的含量，影响了大脑中Paeoniflorin的清除率，抑菌时间的钙霉素-7-O-β-D-6-葡糖苷和大脑的6-姜醇。在正常和脑缺血再灌注大鼠中，血红素素和6-姜醇的含量高于脑组织中的含量，而钙霉素含量，脑内萼素-7-O-β-D-6-葡糖苷含量组织高于血液中的组织，表明枯牛酸和萝卜霉素-7-O-β-D-6-葡糖苷具有脑靶向性质。

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