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首页> 外文期刊>Journal of Diabetes and Metabolic Disorders >Involvement of fat mass and obesity gene (FTO) in the anti-obesity action of Annona muricata Annonaceae: in silico and in vivo studies
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Involvement of fat mass and obesity gene (FTO) in the anti-obesity action of Annona muricata Annonaceae: in silico and in vivo studies

机译：脂肪质量和肥胖基因（FTO）在Annona Muricata Annonaceae的抗肥胖作用中的参与：在硅和体内研究中

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Background Annona muricata (Annonaceae) known as soursop is a common tropical plant species known for its numerous medicinal properties including obesity. The underlying mechanism of anti-obesity effect of A. muricata was investigated. The fat mass and obesity associated protein (FTO) is a validated potential target for anti-obesity drugs.MethodsThe interaction of compounds previously characterized from A. muricata was investigated against FTO using Autodock Vina. Also, modulation of FTO and STAT-3 mRNA expression by A. muricata was investigated in high fat diet induced obese rats (HFDR) using RT-PCR.ResultsA significant up-regulation of FTO gene was observed in HFDR when compared to control rats, while administration of A. muricata (200?mg/kg) significantly ( p ?0.05) down-regulated FTO gene expression when compared to HFDR group. The effect of obesity on STAT-3 gene expression was also reversed by A. muricata (200?mg/kg). In silico study revealed annonaine and annonioside (?9.2?kcal/mol) exhibited the highest binding affinity with FTO, followed by anonaine and isolaureline (?8.6?kcal/mol). Arg-96 is a critical amino acid enhancing anonaine, isolaureline-FTO binding.ConclusionThis study suggests the possible anti-obesity mechanism of A. muricata is via down-regulation of FTO with concomitant up-regulation of STAT-3 genes. This study confirmed the use of this plant in the management of obesity and the probable compounds responsible for its antiobesity effect are annonaine and annonioside.
机译：背景技术Annona Muricata（Annonaceae）被称为Soursop是一种常见的热带植物物种，其众多药用特性包括肥胖症。研究了宫内数据的抗肥胖效应的潜在机制。脂肪质量和肥胖相关蛋白（FTO）是抗肥胖药物的验证潜在的靶标.，使用Autodock Vina针对FTO研究了先前特征的化合物的相互作用。此外，在高脂肪饮食诱导的肥胖大鼠（HFDR）中研究了FTO和STAT-3 mRNA表达的调节，使用RT-PCR.Resultsa与对照大鼠相比，在HFDR中观察到FTO基因的显着上调，与HFDR组相比，在施用A. muricata（200μlmg/ kg）（p <0.05）下调的FTO基因表达。肥胖症对STAT-3基因表达的影响也被A.Muricata（200？Mg / kg）逆转。在硅研究中，揭示了Annonaine和Annonioside（αkcal / mol），其与FTO具有最高的结合亲和力，其次是Anonaine和IsolaReline（？8.6？Kcal / mol）。 Arg-96是一种临界氨基酸增强Anonaine，IstOreline-FTO结合。结论该研究表明A.Muricata的可能抗肥胖机制是通过对FTO的下调，伴随统计数据的上调。本研究证实了这种植物在肥胖症管理中的使用，并且负责其抗病效应的可能化合物是胭脂蛋白酶和环保糖苷。

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