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首页> 外文期刊>Journal of genetics >Molecular characterization of PgUFGT gene and R2R3-PgMYB transcription factor involved in flavonoid biosynthesis in four tissues of wild pomegranate (Punica granatum L.)
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Molecular characterization of PgUFGT gene and R2R3-PgMYB transcription factor involved in flavonoid biosynthesis in four tissues of wild pomegranate (Punica granatum L.)

机译：Pufgt基因的分子表征和野生石榴四种组织（Punica Granatum L.）中黄酮类生物合成中的R2R3-PGMYB转录因子

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The diversity on fruit colouration in plants directly depends on the flavonoids that explain the development of different pigmentation patterns. Anthocyanins are the major class of flavonoid pigments that are synthesized through flavonoid biosynthetic pathway. In the present study, two genes: PgUFGT gene and R2R3-PgMYB gene, involved in anthocyanin biosynthesis were analysed in four tissues of wild pomegranate. The structural genes, UDP-glucose: flavonoid-3-O-glucosyl transferase (PgUFGT; GenBank accession number: MK058491) and its myeloblastosis transcription factor (R2R3-PgMYB; GenBank accession number: MK092063) were isolated and their expression pattern were studied. Molecular modelling indicated that the main secondary structures of PgUFGT and R2R3-PgMYB genes are ?±-helix and random coil. In addition, expression profiling of PgUFGTand R2R3-PgMYB by quantitative-real time PCR indicated a positive correlation between anthocyanin content and their expression in leaves, flowers, green and red fruits of wild pomegranate. Among all the tissues, the red fruit exhibited high transcripts levels of PgUFGT as well as R2R3-PgMYB transcription factor. An extensive homology with UFGTs from other plants was revealed on comparative and bioinformatic analyses. Present study reveals that PgUFGT plays a predominant role in anthocyanin content in wild pomegranate fruits. Further, it is strongly suggested that R2R3-PgMYB transcription factor regulates the anthocyanin biosynthesis in wild pomegranate via expression of PgUFGT gene. This is the first study which provides an insight on expression profile of PgUFGT and R2R3-PgMYB that are involved in colour development and fruit ripening in wild pomegranate.
机译：植物中果实着色的多样性直接取决于解释不同色素沉着模式的发展的黄酮类化合物。花青素是通过黄酮类生物合成途径合成的主要类黄酮颜料。在本研究中，在野生石榴的四个组织中分析了两个基因：Pufgt基因和参与花青素生物合成的R2R3-PGMyB基因。分离出分离出分离出来的结构基因，UDP-葡萄糖：黄酮-3-O-葡萄糖酰转移酶（Guf Genbank登录号：MK058491）及其肌脲母细胞分离转录因子（R2R3-PGMYB; GenBank登录号：MK092063），研究了它们的表达模式。分子建模表明pGufgt和R2R3-PGMYB基因的主要二次结构是α-ε和随机线圈。此外，通过定量实时PCR的Pufgtand R2R3-PGMyb的表达分析表明，野生石榴的叶子，花，绿色和红色果实中的花青素含量与其表达之间的正相关性。在所有组织中，红色果实表现出pufgt以及R2R3-PGMYB转录因子的高转录物水平。在比较和生物信息分析上揭示了来自其他植物的UFGTS的广泛同源性。目前的研究表明，Pufgt在野生石榴果实中的花青素含量中起着主要作用。此外，强烈建议R2R3-PGMYB转录因子通过Pufgt基因的表达调节野生石榴中的花青素生物合成。这是第一项研究，了解Pufgt和R2R3-PGMYB表达谱的洞察，这些研究涉及野生石榴的颜色开发和果实成熟的。

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